# MYOFIBRIL ASSEMBLY IN STRIATED MUSCLE

> **NIH NIH R01** · WEILL MEDICAL COLL OF CORNELL UNIV · 1992 · $258,292

## Abstract

The longterm goal of this project is to define the post-
translational steps in the assembly and remodeling of myofibrils
of cross-striated muscle.  This organelle exhibits characteristic
isoform replacements during the course of development and its
proteins undergo constant turnover throughout life.  Muscle can
increase in diameter in response to exercise (work-induced
hypertrophy) or atrophy with disuse or in response to protein
malnutrition.  Myofibrils constitutes the major amino acid store
of the body and these can be mobilized for the maintenance of
other essential organs, eg. brain, liver, heart, kidneys, etc.,
upon starvation.  We propose to test the hypothesis that
polymerized proteins of the sarcomere are in dynamic equilibrium
with monomers in the cytoplasm: compositional changes of the
myofibrils result from insertion of new proteins into preexisting
fibrils rather than the en bloc destruction and reassembly of
whole sarcomeres or myofilaments.  Seven sets of experiments are
planned.  1.  In vitro analysis of myofibrillar protein exchange.
2.  In vivo analysis of myofibrillar protein pools.  3.
Identification and analysis of myosin-binding proteins which
regulate thick filament assembly and subunit exchange.  4.
Cloning and primary structure determination of muscle C-protein
and 86 kD protein.  5. Molecular genetic dissection of the
protein domains in myosin required for polymerization and
sarcomerogenesis.  6. Analysis of actin isoforms in
myofibrillogenesis.  7. Genetic analysis of sarcomere assembly by
DNA-mediated gene transfer experiments.  These experiments will
form the basis of future studies in man directed at the
pathogenesis of muscle wasting in myopathies, cachexia associated
with neoplasia and aging, and in severe burns.

## Key facts

- **NIH application ID:** 3156206
- **Project number:** 5R01AR032147-11
- **Recipient organization:** WEILL MEDICAL COLL OF CORNELL UNIV
- **SAM.gov UEI:** YNT8TCJH8FQ8
- **Principal Investigator:** DONALD A FISCHMAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1992
- **Award amount:** $258,292
- **Award type:** 5
- **Project period:** 1982-07-01 → 1994-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3156206

## Citation

> US National Institutes of Health, RePORTER application 3156206, MYOFIBRIL ASSEMBLY IN STRIATED MUSCLE (5R01AR032147-11). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3156206. Licensed CC0.

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