# POST-RIBOSOMAL PROTEOGLYCAN MODIFICATION

> **NIH NIH R01** · UNIVERSITY OF MINNESOTA · 1989 · $123,505

## Abstract

The role of phosphorylation of proteoglycans in the intracellular sorting
and control of biosynthesis of these molecules will be investigated using
tissue culture, radioisotopic labeling and detailed chemical analysis.  The
detailed structure of the modified linkage region for the Swarm rat
chondrosarcoma chondroitin sulfate proteoglycan and the EHS heparan sulfate
proteoglycan will be determined using such methods as enzymatic digestions,
methylation, GC-mass spectrophotometry and 31 P NMR.  Enzymatic digestions
will also be utilized to identify the sequence of phosphorylated peptides,
and, to order the substitution sites with the proteoglycan.  The
phosphorylating enzymes, including the 2-phosphoxylose phosphorylating
enzyme will be characterized as to donor and substrate specificity, size,
location and chemical composition.  The subcellular distribution of
modifying enzyme(s) will be determined by standard subcellular
fractionation and analysis.  In addition, the regulation of the
modification will be examined with cultured cells perturbed by reagents
such as insulin, calmodulin inhibitors and cyclic AMP altering drugs, as
well as by monesin and other blockers of intracellular transport.

## Key facts

- **NIH application ID:** 3156279
- **Project number:** 5R01AR032372-07
- **Recipient organization:** UNIVERSITY OF MINNESOTA
- **SAM.gov UEI:** KABJZBBJ4B54
- **Principal Investigator:** Theodore Richard Oegema
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $123,505
- **Award type:** 5
- **Project period:** 1983-07-01 → 1991-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3156279

## Citation

> US National Institutes of Health, RePORTER application 3156279, POST-RIBOSOMAL PROTEOGLYCAN MODIFICATION (5R01AR032372-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3156279. Licensed CC0.

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