# SYNTHESIS OF VITAMIN D2 METABOLITES & RELATED COMPOUNDS

> **NIH NIH R01** · UNIVERSITY OF WISCONSIN-MADISON · 1987 · $61,748

## Abstract

A continuation of synthetic chemical work relating to the vitamin D2
metabolite series is proposed.  Planned new efforts are based directly on
progress made thus far which includes the development of synthetic routes
to 25-hydroxyvitamin D2 and 1a, Alpha25-dihydroxyvitamin D2 and their
corresponding 24R-methyl epimers.  Hence, proposed continued work focuses
on exploiting this method development effort by preparing larger amounts of
these metabolites and their epimers, and by synthesizing the corresponding
tritium-labeled forms.  In addition, the preparation of certain sidechain
analogs of the natural products (modified at C-24 and/or at the double-bond
site) to explore the effect of distinct structural parameters on the
expression of biological activity.  Since available bioassay results have
shown the synthetic 24R-epimer of (1alpha,25-(OH)2D2 to exhibit
differential activity in intestine vs. bone, detailed biological assays
(including bone histological measurements) and some metabolism work is
planned to explore and explain this unique phenomenon.  All other analogs
synthesized will also be tested in rats and chicks, to obtain data on the
potency of these compounds in stimulating intestinal calcium and phosphate
transport, bone mineral mobilization, and bone mineralization.  The
activity of synthetic vitamin D2 compounds in promoting the differentiation
human myeloid leukemia cells will also be investigated in human
promyelocytic HL-60 cell cultures.

## Key facts

- **NIH application ID:** 3156382
- **Project number:** 5R01AR032701-05
- **Recipient organization:** UNIVERSITY OF WISCONSIN-MADISON
- **SAM.gov UEI:** LCLSJAGTNZQ7
- **Principal Investigator:** HECTOR F DE LUCA
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $61,748
- **Award type:** 5
- **Project period:** 1983-08-01 → 1988-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3156382

## Citation

> US National Institutes of Health, RePORTER application 3156382, SYNTHESIS OF VITAMIN D2 METABOLITES & RELATED COMPOUNDS (5R01AR032701-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3156382. Licensed CC0.

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