# MYOSIN LINKED REGULATION IN MUSCLE AND NON-MUSCLE CELLS

> **NIH NIH R01** · ALLEGHENY UNIVERSITY OF HEALTH SCIENCES · 1989 · $176,808

## Abstract

This proposal is aimed at furthering an understanding of the mechanism and
function of myosin-linked regulation, both in muscle and non-muscle cells.
In regulatory myosins, the interaction between actin and myosin is
controlled solely by events occurring on the myosin molecule alone, either
in response to direct calcium binding or phosphorylation.

We will take advantage of the unique and versatile properties of scallop
striated adductor myosin in order to incorporate foreign light-chains, both
regulatory and essential, into the myosin molecule.  These light-chains
will be modified at differing locations in their primary sequence.  Our
experiments will monitor intra-head conformational changes by fluorescence
resonance energy transfer (FRET).  These events will be manifest as changes
in distance and/or orientation between probes placed on each of the two
light-chain types and between probes on either light-chain and the active
site, or other locations on both actin and myosin.  Changes in the degree
of transfer will be sought in response to changing conditions designed to
mimic the physiological states of 'rest', 'rigor' or the 'active state.'
The complementary technique of protein crosslinking will also be used, when
appropriate.

We also intend to perform a comparative study of two regulatory myosins
which exhibit both light-chain and heavy-chain phosphorylation and to
observe the relationship of such regulation to the specific functions of
myosin in situ.  By using molluscan catch adductor myosin we will further
our understanding of the regulation of catch contraction.  By using myosin
isolated from a neuronally-derived cell line, we will ultimately be able to
evaluate the role of myosin in neurotransmitter release during synaptic
transmission.  An antibody to this myosin will enable us to probe the
location and function of myosin within the developing central nervous
system.

A detailed understanding of these mechanisms is of fundamental importance.

## Key facts

- **NIH application ID:** 3156428
- **Project number:** 2R01AR032858-04A2
- **Recipient organization:** ALLEGHENY UNIVERSITY OF HEALTH SCIENCES
- **Principal Investigator:** PETER D CHANTLER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $176,808
- **Award type:** 2
- **Project period:** 1983-07-01 → 1993-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3156428

## Citation

> US National Institutes of Health, RePORTER application 3156428, MYOSIN LINKED REGULATION IN MUSCLE AND NON-MUSCLE CELLS (2R01AR032858-04A2). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3156428. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*