# THE IMMUNOPATHOLOGY OF PEMPHIGUS AND BULLOUS PEMPHIGOID

> **NIH NIH R01** · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · 1989 · $153,517

## Abstract

The objectives are to continue to investigate immunopathogenic mechanisms
in pemphigus and bullous pemphigoid.  We have recently presented evidence
that complement is fixed to epidermal cell surfaces by pemphigus antibody,
that complement enhances pemphigus IgG mediated detachment of epidermal
cells, and that complement in the presence of pemphigus IgG alters the
integrity of epidermal cell membranes.  Under Aim I, we hope to study
further this complement mediated attack upon the epidermal cell by
immunofluorescent (IF), immunoelectron microscopy (IEM), and by cell
detachment assays.  We hope to determine which component(s) is involved in
the process and if complement activation enhances the release of
plasminogen activator.  As pemphigoid antibody reacts with the polar region
of basal cells, we would also like to propose to determine if complement
will damage basal cell membranes as well (Aim II).  Our last aim is to
continue to purify the antigens involved in both disease processes by
affinity chromatography so that we may purify the antibodies for studies of
complement mediated damage, gene isolation and IEM studies.  Monoclonal
antibodies will be produced which will be utilized in all of the studies
mentioned above, in addition to further purification of epidermal
antigens.  Since so little is known concerning the killing of nucleated
cells by complement, these two disease processes, with specific cells as
targets, offer unique opportunities to study this mechanism.

## Key facts

- **NIH application ID:** 3156475
- **Project number:** 5R01AR032974-07
- **Recipient organization:** UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
- **SAM.gov UEI:** ZUFBNVZ587D4
- **Principal Investigator:** ROBERT E JORDON
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $153,517
- **Award type:** 5
- **Project period:** 1983-07-01 → 1991-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3156475

## Citation

> US National Institutes of Health, RePORTER application 3156475, THE IMMUNOPATHOLOGY OF PEMPHIGUS AND BULLOUS PEMPHIGOID (5R01AR032974-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3156475. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*