# STRUCTURE OF THE Z DISK OF STRIATED MUSCLE

> **NIH NIH R01** · UNIVERSITY OF ARIZONA · 1989 · $150,508

## Abstract

The objective of this project is to determine the 3-D arrangement
of actin filaments and auxiliary proteins in an actin anchoring
network, the Z disk of striated muscle.

The analysis will be based on electron microscopy, applied in
combination with 3-D image reconstruction and averaging techniques.
3-D images (density maps) of native Z disks will be calculated from
images of tilted thin sections; these will be analyzed to determine
the arrangements of components and their interconnections.

(1)  The structure analysis of the entire bee flight muscle Z disk
will be completed by determining the structure of the flanking
regions of the Z disk which were not included in the
reconstructions done in the previous grant period.  The structure
of the attachment site of C filament extensions of the thick
filament to the Z line will be determined.
(2)  Structure features resolved in Z disk density maps will be
identified by specific antibody labelling of bee Z disks, followed
by 3-D structure analysis.  Antibodies to alpha-actinin, and other
Z disk component proteins will be prepared and used as labels.
(3)  The dynamics and function of the Z disk structure will be
analyzed by determining the 3-D structure of the Z disk in
different states of bee flight muscle contraction and tension.
(4)  The 3-D structure of the Drosophila Z disk will b determined
as a first step towards a combined genetic and structural analysis
of the function of the component proteins of the Z disk.
(5)  Pilot labelling experiments with monoclonal antibodies raised
against Z disks and their component proteins will be carried out,
with the eventual goal of identifying and localizing all of the
component proteins of the Z disk.

## Key facts

- **NIH application ID:** 3156518
- **Project number:** 2R01AR033141-04A1
- **Recipient organization:** UNIVERSITY OF ARIZONA
- **SAM.gov UEI:** ED44Y3W6P7B9
- **Principal Investigator:** JAMES F DEATHERAGE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $150,508
- **Award type:** 2
- **Project period:** 1983-12-01 → 1994-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3156518

## Citation

> US National Institutes of Health, RePORTER application 3156518, STRUCTURE OF THE Z DISK OF STRIATED MUSCLE (2R01AR033141-04A1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3156518. Licensed CC0.

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