# HLA ANTIGEN AND DNA RESTRICTION SITE DISEASE MARKERS

> **NIH NIH R01** · STANFORD UNIVERSITY · 1987 · $175,357

## Abstract

Two hypotheses proposed to explain observed relationships between HLA
antigens and disease susceptibility are either that the antigens themselves
play some direct role in pathogenesis or they are merely fortuitous markers
for nearby specific disease susceptibility genes.  This proposal seeks data
to distinguish between these hypothesis by using molecular probes to
directly characterize the DNA of the HLA region, particularly comparing
controls and patients with autoimmune diseases.  DNA from peripheral blood
lymphocytes will be digested with restriction endonucleases, separated
electrophoretically by size on agarose gels, and transfered to
nitrocellulose filters for hybridization with 32P labeled, cloned HLA gene
probes.  Locus and allele specific HLA class I probes will be sought from
non-homologous intragenic and flanking regions of cloned HLA class I genes,
either by excision and isolation or by synthesis of the desired
oligonucleotide sequences.  Currently available and new probes will be
tested against normal controls, HLA recombinant families, and cloned
genomic HLA genes (as markers) to characterize normal HLA class I
restriction fragment length polymorphism (RFLP) and its correlation with
known HLA antigens.  De novo fragment generation has been observed and will
also be investigated, ultimately by cloning and sequencing the novel and
parental fragments.  RFLP of variant B27 (+) normals and B27 (+)
spondyloarthropathy patients will be compared to seek disease specific
markers.  Identification of such markers should permit identification of
the pertinent gene products and ultimately clarification of the mechanism
of pathogenesis.

## Key facts

- **NIH application ID:** 3156534
- **Project number:** 5R01AR033233-04
- **Recipient organization:** STANFORD UNIVERSITY
- **SAM.gov UEI:** HJD6G4D6TJY5
- **Principal Investigator:** FRANK CARL GRUMET
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $175,357
- **Award type:** 5
- **Project period:** 1984-07-01 → 1988-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3156534

## Citation

> US National Institutes of Health, RePORTER application 3156534, HLA ANTIGEN AND DNA RESTRICTION SITE DISEASE MARKERS (5R01AR033233-04). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3156534. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*