# MONOCYTE/NEUTROPHIL OXIDANTS AND LYMPHOCYTE FUNCTION

> **NIH NIH R01** · UNIVERSITY OF MINNESOTA · 1988 · $111,490

## Abstract

The long-term objective is to examine interactions betwen activated
phagocytes and human lymphocytes.  These studies are based on the premise
that in situations of chronic inflammation, activated phagocytes releasse
mediators such as certain prostaglandins and reactive oxygen species and
thereby influence the subtype distribution and function of nearby
lymphocytes.

Our previous studies clearly showed that oxidants such as hydrogen peroxide
(H2O2) when released from activated blood phagocytes inhibit in vitro T
cell rosette formation, T cell proliferation, and mononuclear cell
production of immunoglobulin.  Preliminary data strongly indicate that T
cells are not equally sensitive to physiological amounts of H2O2.  For T
cell-sheep erythrocyte rosette formation, there exists discrete peroxide
sensitive and resistant T cell subpopulations with markedly different T
cell subset distributions and proliferative functions.  Oxidants other than
H2O2, such as hypochlorous acid and N-chloroamines also inhibit T
cell-rosette formation in non-lethal amounts.

The present studies will examine in detail the effects of three
phagocyte-released oxidants, H2O2, hypochlorous acid and N-chlorotauramine,
on several important interrelated lymphocyte functions.  Oxidants will be
either added directly to lymphocytes or generated in vitro with activated
phagocytes in the presence of selective oxygen radical scavengers.
Specific oxidant effects on T cell rosette formation, T cell subset
distribution, T cell subset and B cell proliferation, lymphokine production
and response, and T cell subset regulation of B cell immunoglobulin
production will be examined.  T cells highly resistant to different
oxidants will be developed through multiple exposure of activated
lymphocytes to low level oxidants.  Monoclonal antibodies will be produced
against lymphocytes particularly sensitive or resistant to different
oxidants for use as markers and reagents for cell separation in a more
refined in vitro analysis and for in vivo detection purposes at chronic
inflammatory sites.

Data gathered from these studies should provide important insights as to
how inflammatory mediators can alter in vitro immune reactivity and may
provide important probes for further investigations of phagocyte-lymphocyte
interaction in vivo.

## Key facts

- **NIH application ID:** 3156573
- **Project number:** 5R01AR033492-02
- **Recipient organization:** UNIVERSITY OF MINNESOTA
- **SAM.gov UEI:** KABJZBBJ4B54
- **Principal Investigator:** DAVID C ZOSCHKE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $111,490
- **Award type:** 5
- **Project period:** 1986-12-01 → 1989-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3156573

## Citation

> US National Institutes of Health, RePORTER application 3156573, MONOCYTE/NEUTROPHIL OXIDANTS AND LYMPHOCYTE FUNCTION (5R01AR033492-02). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3156573. Licensed CC0.

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