# ISCHEMIC INJURY IN SKIN FLAPS: MECHANISM AND PREVENTION

> **NIH NIH R01** · JOHNS HOPKINS UNIVERSITY · 1987 · $148,189

## Abstract

The major consideration in utilizing island flaps and free flaps of skin
and subcutaneous tissue is tissue viability.  Superoxide free radicals have
been implicated in the etiology of cellular injury in various
pathophysiological processes.  The present proposal seeks to elucidate the
role of superoxide free radicals in the tissue damage which occurs during
ischemia and reperfusion.

Allopurinol blockade of superoxide generation and scavengers of oxygen free
radicals (superoxide dismutase, dimethyl sulfoxide, Alpha-tocopherol) will
be investigated in terms of enhanced flap survival.  The effect of
hyperbaric oxygen on flap survival will be investigated to determine
possible induction of antitoxidant enzymes.  Additionally, the glutathione
peroxidase system and reducing equivalents (NADH, NADPH, GSH) will be
investigated.

Fundamental knowledge concerning detoxification of free radicals will
suggest therapeutic means for minimizing the effects of ischemia.  Insights
gained with respect to free flaps will prove valuable in the field of
allotransplantation.

## Key facts

- **NIH application ID:** 3156605
- **Project number:** 5R01AR033638-03
- **Recipient organization:** JOHNS HOPKINS UNIVERSITY
- **SAM.gov UEI:** FTMTDMBR29C7
- **Principal Investigator:** JOHN E HOOPES
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $148,189
- **Award type:** 5
- **Project period:** 1985-09-20 → 1989-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3156605

## Citation

> US National Institutes of Health, RePORTER application 3156605, ISCHEMIC INJURY IN SKIN FLAPS: MECHANISM AND PREVENTION (5R01AR033638-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3156605. Licensed CC0.

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