# UV RADIATION AND EPIDERMAL MEMBRANE MESSENGER SYSTEMS

> **NIH NIH R01** · COLUMBIA UNIV NEW YORK MORNINGSIDE · 1993 · $191,953

## Abstract

DESCRIPTION (adapted from investigator's abstract and/or aims):  This is a
revised renewal application to study the effect of UV radiation on certain
aspects of epidermal keratinocyte membrane messenger systems.  The major
focus of the study is based on the fact that solar ultraviolet radiation is
a ubiquitous environmental agent known to produce numerous effects in human
skin including inflammation, pigmentation, alteration of immune function
and carcinogenesis.  The mechanism by which solar radiation evokes most
cutaneous photobiological responses remains poorly defined.  Specifically,
little attention has been paid to the possible role of membrane
constituents in mediation of UV-induced skin changes.  Based on
epidemiological studies, it is known that UV-induced skin damage results in
stimulation of many biologic processes analogous to changes induced by
membrane active agents - hormones, growth factors and chemical
tumor-promoters.  These agents are known to act through alteration in
membrane structure and function including phospholipase activation and
membrane receptor/kinase activation, resulting in the activation of second
messenger signals.  In turn, these second messenger systems play a crucial
role in regulation of cell growth and differentiation in both normal skin
and in skin diseases such as psoriasis and cancer.  The overall objective
of the proposed studies is to examine the effect of UV radiation on
selected aspects of membrane messenger system metabolism in human
epidermis.  The four specific aims are to utilize human keratinocytes in
culture as a model to assay the effect of UV radiation (UVB-290-320 nm;
UVA-302-400 nm) on phospholipase activation and diacylglycerol (DAG)
production, protein kinase C (PKC) abundance and activity, alterations of
binding of epidermal growth factor (EGF) to its receptor.  Finally, the
investigator will assay cellular proliferation by labelled thymidine
incorporation and cell counting; and cellular differentiation by envelope
counting.  It is proposed that the delineation of the effect of UV on
membrane messenger system metabolism of human keratinocytes should yield
insight into the role played by such effects on the overall tissue response
of human skin to sunlight, extending the knowledge of how UV radiation
induces deleterious effects in normal skin and positive effect in diseased
skin as occurs in phototherapy of psoriasis.

## Key facts

- **NIH application ID:** 3156615
- **Project number:** 5R01AR033663-09
- **Recipient organization:** COLUMBIA UNIV NEW YORK MORNINGSIDE
- **SAM.gov UEI:** F4N1QNPB95M4
- **Principal Investigator:** VINCENT A DELEO
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $191,953
- **Award type:** 5
- **Project period:** 1984-04-01 → 1994-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3156615

## Citation

> US National Institutes of Health, RePORTER application 3156615, UV RADIATION AND EPIDERMAL MEMBRANE MESSENGER SYSTEMS (5R01AR033663-09). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3156615. Licensed CC0.

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