# CONTRACTION OF SKINNED FIBERS OF CARDIOMYOPATHY

> **NIH NIH R01** · ALBERT EINSTEIN COLLEGE OF MEDICINE · 1988 · $201,046

## Abstract

The overall goals of our studies are to advance the understanding
of the cross-bridge mechanisms in muscular contraction, and to
provide insights into the relationships between the biochemical and
mechanical events in the cross-bridge cycle.  To achieve the
major goals chemically treated skinned hamster fiber preparations
are utilized having certain moities extracted from the contractile
apparatus and then reconstituted with either homologous proteins
of the same tissue or the heterologous moities such as from
cardiac muscle or from other species.  In addition the intact fiber
preparation from frog muscle is used in selected experiments to
duplicate conditions closer to in vivo.  To study properties of the
cross-bridge mechanisms, the experiments on control fibers are
carried out at various ionic strengths which helps isolate the
individual states of cross-bridges that are representative of the
configurations during the normal cross-bridge cycle.  The specific
aims are to (1) identify the steps in the cycle that are regulated
by Ca2+, (2) specify the roles of the regulatory proteins with
special emphasis on the identification and characterization of
presumably a new cofactor in muscle regulation, and (3) study of
the rate limiting steps in the cross-bridge cycle of Vmax and
force development.  The study is also made of (4) the modification
of the cross-bridge kinetics by internal loads that are increased by
the compressions of the filament lattice and specifically examines
whether the origin of filamentory interactions during such
compressions is from particular cross-bridge configurations.  The
implications of these cross-bridge configurations for the normal
contraction and relaxation is examined.  In addition (5) the
observations on the compartmentalizations of calmodulin in
muscle fiber are extended to examine the location of CaM in the
fibers by using the immunological techniques combined with EM.
The specific roles of the various Ca2+ binding sites in the
different aspects of the activation of cross-bridges for
contraction are also examined by using different Ca-binding
proteins.  The results would advance the understanding of the
regulatory mechanisms in the contraction process as well as
provide new insights into the cross-bridge mechanisms.

## Key facts

- **NIH application ID:** 3156641
- **Project number:** 2R01AR033736-04A1
- **Recipient organization:** ALBERT EINSTEIN COLLEGE OF MEDICINE
- **Principal Investigator:** JAGDISH GULATI
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $201,046
- **Award type:** 2
- **Project period:** 1984-12-01 → 1991-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3156641

## Citation

> US National Institutes of Health, RePORTER application 3156641, CONTRACTION OF SKINNED FIBERS OF CARDIOMYOPATHY (2R01AR033736-04A1). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3156641. Licensed CC0.

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