# T CELL CONTROL OF AUTOREACTIVITY IN MURINE SLE

> **NIH NIH R01** · UNIV OF NORTH CAROLINA CHAPEL HILL · 1992 · $195,952

## Abstract

Although T cells are intimately involved in the development of
murine systemic lupus erythematosus (SLE), the understanding of
their role in disease has been elusive.  Our previous studies of T-
cell reactivity to the Sm nuclear antigen have provided a system
to investigate T-cell recognition of an antigen important in SLE.
we will define the T-cell receptors utilized by Sm-reactive T cells
by creating T-cell hybridomas reactive to mouse Sm, identifying
the T-cell receptor V gene families, and sequencing the genes so
that we can compare the hybridomas to each other and to
germline genetic elements.

Lpr is  a single recessive gene which causes vast expansion of a
population of L3T4- LYT-2- ("double negative") T lymphocytes in
the periphery.  We wish to understand how these T cells provoke
autoimmune disease and thus will explore their origin and
differentiative capacity through adoptive transfer experiments
using Ly 1 congenic lpr mice.  We will further define this disease-
related double negative lineage using monoclonal antibodies
directed against thymic double negative cells.  We will investigate
the surface receptors and function of hybridomas generated from
the lpr double negative cells to gain understanding of these cells
at the clonal level.  By adoptive transfer of lpr and normal
marrow into doubly heterozygous lpr hosts, we will ask whether
the prominent B cell and macrophage abnormalities characteristic
of lpr mice are due to the action of the abnormal T cells or
instead reflect intrinsic abnormalities in other bone marrow
derived lineages.  Finally, we will exploit the capacity of lpr to
induce autoimmunity on diverse murine backgrounds to determine
the influence of major histocompatibility class I and class II
genes on expression of SLE.

## Key facts

- **NIH application ID:** 3156673
- **Project number:** 5R01AR033887-08
- **Recipient organization:** UNIV OF NORTH CAROLINA CHAPEL HILL
- **SAM.gov UEI:** D3LHU66KBLD5
- **Principal Investigator:** PHILIP L COHEN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1992
- **Award amount:** $195,952
- **Award type:** 5
- **Project period:** 1984-12-01 → 1992-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3156673

## Citation

> US National Institutes of Health, RePORTER application 3156673, T CELL CONTROL OF AUTOREACTIVITY IN MURINE SLE (5R01AR033887-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3156673. Licensed CC0.

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