# CRYSTALLOGRAPHIC STUDIES OF TROPONIN C

> **NIH NIH R01** · UNIVERSITY OF WISCONSIN-MADISON · 1989 · $112,945

## Abstract

The main focus of this research will be to:  Extend the refinement
studies on CaTNC from chicken skeletal muscle from 2.0 alpha to
1.7 alpha resolution; complete the refinement of the isomorphous
MnTNC structure at 2.2 alpha resolution; extend the data of Cd and
NdTNC to higher resolution for refinement; prepare MgTNC and the
four-calcium state TNC for crystallization and x-ray structure
analysis; seek optimum conditions to improve the quality and size
of the initial crystals that we have obtained on bovine cardiac TNC
(which binds only three calcium ions) for possible x-ray structure
determination; carry out crystallization of the multiprotein
complexes the whole troponin C + I + T, and C + I.  Besides the
crystallographic work, quantitative structure-activity relationship
studies (QSAR) will be conducted relating binding constants, ligand
charge, hydrophobicity of helices and beta-sheets; in addition, the
role of charged side chains on the stability of alpha-helices
exposed to solvent will continue to.be investigated.  We have made
important advances in many of these studies.  The x-ray and QSAR
studies are providing a wealth of information on the structural
principles governing the calcium-binding proteins and the nature
of the forces controlling the calcium binding affinities.  The high
resolution structural information on the troponin family of muscle
proteins will help towards a better understanding of the molecular
mechanism of muscular contraction and thus provide insights on
various disease states.

## Key facts

- **NIH application ID:** 3156739
- **Project number:** 5R01AR034139-05
- **Recipient organization:** UNIVERSITY OF WISCONSIN-MADISON
- **SAM.gov UEI:** LCLSJAGTNZQ7
- **Principal Investigator:** MUTTAIYA SUNDARALINGAM
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $112,945
- **Award type:** 5
- **Project period:** 1984-08-01 → 1991-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3156739

## Citation

> US National Institutes of Health, RePORTER application 3156739, CRYSTALLOGRAPHIC STUDIES OF TROPONIN C (5R01AR034139-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3156739. Licensed CC0.

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