# ROLE OF METABOLIC FACTORS IN BONE FORMATION

> **NIH NIH R01** · UNIVERSITY OF PENNSYLVANIA · 1988 · $149,145

## Abstract

The major thrust of this research proposal is to examine the role
of energy metabolism in the initiation of mineralization of the
epiphyseal growth cartilage.  We will test the hypothesis that a
change in energy metabolism results in phosphate and calcium
fluxes leading to mineral deposition.  Utilizing innovative high
resolution image processing techniques we will generate
metabolic maps of the growth cartilage and relate cell redox,
adenine nucleotide levels and oxygen tension, at the cytochromes,
to maturation and calcification of the cartilage.  These maps will
permit us to explore the influence of the vascular canals of the
growth cartilage on chondrocyte metabolism and the development
of mineralization.  We will also relate energy status of the cells
to their mineral content.  We will use cell cultures to determine
how calcium and phosphate ions and vitamin D control the redox
status of chondrocytes.  For this purpose, culture systems will be
used in which we can monitor matrix vesicle biogenesis and
chondrogenesis; these functions will be related to energy
metabolism of the cells, vesicle formation and mineralization.
Another goal of the proposal is to ascertain whether changes in
redox and energy charge lead to changes in membrane lipids; we
will measure membrane lipids and the generation of inositol
phosphates by the growth cartilage can cells in culture and
examine their influence on cell calcium transport.  Finally, we
will also determine how energy metabolism modulates membrane
permeability and potential.  This will be achieved using dyes to
probe plasma and mitochondrial membranes.  Further, we will
measure cytosolic calcium concentrations and relate ion flux to
membrane potential, cell redox, energy metabolism and
mineralization.

## Key facts

- **NIH application ID:** 3156840
- **Project number:** 2R01AR034411-04A1
- **Recipient organization:** UNIVERSITY OF PENNSYLVANIA
- **SAM.gov UEI:** GM1XX56LEP58
- **Principal Investigator:** IRVING M SHAPIRO
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $149,145
- **Award type:** 2
- **Project period:** 1984-07-01 → 1992-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3156840

## Citation

> US National Institutes of Health, RePORTER application 3156840, ROLE OF METABOLIC FACTORS IN BONE FORMATION (2R01AR034411-04A1). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3156840. Licensed CC0.

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