# PROTEIN PHOSPHORYLATION IN MUSCLE

> **NIH NIH R01** · UNIVERSITY OF ILLINOIS AT CHICAGO · 1988 · $96,109

## Abstract

We wish to study the phosphorylation and dephosphorylation of various proteins
of functionally different muscles in order to shed light on the molecular events
of muscle contraction.  Particular aspects of this long-range research aim are:
Changes in the phosphate content of myosin light chains, tropomyosin,
troponin-T, the 10,000 dalton acid chloroform-methanol soluble protein, and the
proteins of the sarcoplasmic reticulum will be determined during a single
isotonic or isometric tetanus of electrically stimulated frog muscle, electrical
stimulation of the stretched muscle, and chemically induced contractures of the
muscle.
We plan to study the effect of denervation, neurotransmitters, and various drugs
on the turnover of protein-bound phosphorus in skeletal muscle and the effect of
inotropic agents on the phosphorylation of tropomyosin in perfused heart.

We plan to compare the phosphorylation of tropomyosin in normal and hereditary
dystrophic muscle.
We plan to apply phosphorus nuclear magnetic resonance for the detection of
phosphoryl tropomyosin and for the detection of changes in the environment of
the tropomyosin-bound phosphate as a result of its interaction with the troponin
complex and actomyosin.
We plan to isolate the enzymes involved in the turnover of the tropomyosin-bound
phosphate.

## Key facts

- **NIH application ID:** 3156874
- **Project number:** 5R01AR034602-14
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT CHICAGO
- **SAM.gov UEI:** W8XEAJDKMXH3
- **Principal Investigator:** MICHAEL BARANY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $96,109
- **Award type:** 5
- **Project period:** 1984-09-01 → 1989-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3156874

## Citation

> US National Institutes of Health, RePORTER application 3156874, PROTEIN PHOSPHORYLATION IN MUSCLE (5R01AR034602-14). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3156874. Licensed CC0.

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