# BIOLOGY OF THE THY-1 ANTIGEN BEARING EPIDERMAL CELLS

> **NIH NIH R01** · UT SOUTHWESTERN MEDICAL CENTER · 1987 · $167,982

## Abstract

The objective of this proposal is to characterize the biologic attributes
of the dendritic Thy-1 antigen-bearing cells which populate mouse epidermis
(EC (Thy-1+)).  Focusing first on their cellular attributes we plan to:
1a) refine methods for obtaining cell populations highly enriched for and
highly depleted of them, 1b) attempt to propagate them in tissue culture
with specialized growth media, 1c) obtain stable cell lines through cell
fusion and viral transformation, 2) complete the phenotypic
characterization of EC(Thy-1+) in adult epidermis and to compare these
attributes with those of other related epidermal cells, 3) assess the
ontogeny and migratory patterns of EC(Thy-1+) during the perinatal period
after skin grafting, and under the influence of specific drugs (a phorbol
ester and corticosteroids), and physical maneuvers (stratum corneum removal
and UVB radiation), and 4) test a series of hypotheses concerning
functional attributes of EC(Thy-1+), including a. They are T lymphocytes,
b. They are non-T lymphocytes, c. They are antigen presenting cells.
Functional properties will be tested in vitro and with in vivo studies in
which EC(Thy-1+) are purified, treated, and reintroduced by various routes
into naive recipients.

## Key facts

- **NIH application ID:** 3157026
- **Project number:** 5R01AR035068-03
- **Recipient organization:** UT SOUTHWESTERN MEDICAL CENTER
- **SAM.gov UEI:** YZJ6DKPM4W63
- **Principal Investigator:** Paul R Bergstresser
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $167,982
- **Award type:** 5
- **Project period:** 1984-12-01 → 1988-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3157026

## Citation

> US National Institutes of Health, RePORTER application 3157026, BIOLOGY OF THE THY-1 ANTIGEN BEARING EPIDERMAL CELLS (5R01AR035068-03). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3157026. Licensed CC0.

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