# CONTROL MODEL FOR HEALING ACROSS A BONE CHAMBER IMPLANT

> **NIH NIH R01** · UNIVERSITY OF SOUTHERN CALIFORNIA · 1988 · $155,565

## Abstract

The primary goal of this project is to establish a quantitative wound
healing model for patterns of reparative osteogenesis, neovascularization
and microcirculation in a disc of tissue regenerating into a bone chamber
which has been implanted in the cortical bone of rabbit tibia medial
plateau.  Chronic observation of the repair process will be analyzed for
differences between this tissue and that which regenerates into the rabbit
ear chamber.  The long term objective which will follow this project is to
perturb this wound healing model with insults which will alter cortical
neovascularization and microcirculation sufficiently to induce a
pathophysiological response to the development and reversal of which will
be observed and quantitatively analyzed.  In future projects this model
will serve as a baseline for studies of the effects of ischemia, infection,
various chemical and electrical agents, etc. on bone repair.

The primary tools for achieving all objectives are the horizontal
intravital microscope and the chronically implanted transcortical bone
chamber.  The general working hypotheses for the norm model are that in the
slit-gap of the bone chamber:

1) The vascular growth pattern is similar to that in the ear chamber until
bony ingrowth commences.

2) As the regenerating bone advances the number and volume of vessels
before the advancing front decrease while the same quantities within the
bone increase.

3) Volumetric flow rates reach a maximum during the week preceding bony
ingrowth.

4) Major blood vessels will tend to align parallel with the long axis of
the main bone after bony ingrowth commences; even if no haversian canals
form.

5) Woven bone precedes lamellar bone during bone front development.

Hypothesis testing will begin by implanting the bone chamber in the tibia.
One week post-op the implanted leg will be mounted in a yoke so as to align
the chamber optical axis with the optical axis of an intravital
microscope.  The healing tissue in the slit field will be recorded on
videotape and still photographs, utilizing simple and polarized
trans-illumination.  Flow pattern observations will include FITC-Dextran
fluorescence under diffused laser epi-illumination.  Observations will
continue weekly through the seventh post-operative week.

Analysis will be performed with an image analysis system which includes a
video position analyzer, frame memory, microcomputer and cross-correlation
velocimeter.

## Key facts

- **NIH application ID:** 3157207
- **Project number:** 5R01AR035473-03
- **Recipient organization:** UNIVERSITY OF SOUTHERN CALIFORNIA
- **SAM.gov UEI:** G88KLJR3KYT5
- **Principal Investigator:** HOWARD WINET
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $155,565
- **Award type:** 5
- **Project period:** 1986-04-01 → 1989-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3157207

## Citation

> US National Institutes of Health, RePORTER application 3157207, CONTROL MODEL FOR HEALING ACROSS A BONE CHAMBER IMPLANT (5R01AR035473-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3157207. Licensed CC0.

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