# MONOCLONAL PROBES OF EPIDERMAL DIFFERENTIATION

> **NIH NIH R01** · ROCKEFELLER UNIVERSITY · 1987 · $128,578

## Abstract

A series of monoclonal antibodies were raised by immunization with a
malignant human lymphoblastoid T cell line.  This panel includes antibodies
specific for differentiation-associated antigens expressed either by basal
keratinocytes; by the stratum corneum and cells in the stratum granulosum;
or by all epidermal keratinocytes.  Further characterization of their
tissue distribution will be performed by immunoperoxidase studies of tissue
sections, and immunofluorescence studies of lymphoid cells and
keratinocytes in vitro.  The functions of these antigens will be
investigated in both epidermal and lymphocyte systems in vitro.
Characterization and purification of these antigens will be done by
SDS-PAGE analysis and affinity chromatography.  These monoclonal antibodies
are being applied to localize abnormalities in keratinization that occur in
diseases of altered epidermal differentiation, e.g., psoriasis.  In
psoriatic skin, evidence has been obtained for abnormal patterns of
epidermal reactivity with our monoclonal antibodies.  These abnormalities
revert to the normal pattern with successful in vivo-administered therapy.
These studies of psoriasis will be enlarged and extended to other diseases
characterized by abnormal epidermal differentiation.  Additionally, these
antibodies will be used to study the effect of retinoids, which are used to
treat psoriasis, pityriasis rubra pilaris and certain of the ichthyosiform
dermatoses, upon in vitro keratinocyte differentiation.  Finally, basal
keratinocyte specific antibodies, which react with cell surface membrane
antigens, will be used to purify basal epidermal keratinocytes.  In vitro
cultures of purified basal keratinocytes will be established and used as
autologous grafts in patients with dystrophic epidermolysis bullosa and
other chronic, nonhealing wounds.

## Key facts

- **NIH application ID:** 3157339
- **Project number:** 5R01AR035676-03
- **Recipient organization:** ROCKEFELLER UNIVERSITY
- **SAM.gov UEI:** LHGDNJMZ64Y1
- **Principal Investigator:** ALICE B GOTTLIEB
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $128,578
- **Award type:** 5
- **Project period:** 1985-09-23 → 1988-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3157339

## Citation

> US National Institutes of Health, RePORTER application 3157339, MONOCLONAL PROBES OF EPIDERMAL DIFFERENTIATION (5R01AR035676-03). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3157339. Licensed CC0.

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