# BONE LOSS AFTER OVARIECTOMY; RESPONSE TO ESTROGEN

> **NIH NIH R01** · UNIVERSITY OF KENTUCKY · 1987 · $116,166

## Abstract

A research program is proposed to continue and to advance investigations
focusing on evolution of histopathological and serum-biochemical changes
associated with loss of ovarian function.  Loss of ovarian function
represents a major contributing factor in the development of postmenopausal
osteoporosis.  The sequelae of osteoporosis constitute one of the greatest
challenge to health and longevity, exceeding cardiovascular-renal diseases
and cancer in prevalence.  In preliminary studies, an in vivo dog model for
bone loss associated with cessation of ovarian function was established in
our laboratory.  This model allows to perform much needed controlled
prospective studies which are not feasible in patients, but closely related
and directly applicable to the bone loss occurring in humans.
Abnormalities in static and dynamic parameters of bone structure and bone
cells occurring during six months after induction of gonadal deficiency
will be unravelled by sequential bone biopsies, mineralized bone histology
and histomorphometry in ovariohysterectomized dogs.  The hormonal
derangements will be elucidated by monthly determinations of sex hormones
including estradiol, progesterone, luteinizing hormone, follicle
stimulating hormone, prolactin and parameters of bone metabolism such as
serum concentrations of calcium, phosphorus, alkaline phosphatase,
parathyroid hormone, bone-Gla-protein, 1.25 Vitamin D3 and urinary
hydroxyproline.  The predictive value of these biochemical parameters for
bone changes associated with loss of ovarian function will be studied.  The
contribution of skeletal mass and total body weight at time of loss of
ovarian function to the rate of bone loss and the amount of bone remaining
after long term ovarian insufficiency will also be studied.  Cancellous and
cortical bone will be separately evaluated to unravel possible differences
in rates or degree of bone loss.  In addition, the role of estrogen
deficiency versus other hormonal derangements in the bone abnormalities
developing after loss of ovarian function will be studied in separate
groups of animals subjected to ovariectomy and subsequent estrogen
replacement.  The studies will fill a most important gap currently existing
in the understanding of evolution of bone loss and the role of estrogen in
the bone abnormalities associated with ovarian insufficiency.

## Key facts

- **NIH application ID:** 3157383
- **Project number:** 5R01AR035837-02
- **Recipient organization:** UNIVERSITY OF KENTUCKY
- **SAM.gov UEI:** H1HYA8Z1NTM5
- **Principal Investigator:** Hartmut H Malluche
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $116,166
- **Award type:** 5
- **Project period:** 1986-04-01 → 1989-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3157383

## Citation

> US National Institutes of Health, RePORTER application 3157383, BONE LOSS AFTER OVARIECTOMY; RESPONSE TO ESTROGEN (5R01AR035837-02). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3157383. Licensed CC0.

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