# GLYCOLYSIS AND MITOCHONDRIA: INTERACTION IN VIVO

> **NIH NIH R01** · UNIVERSITY OF ROCHESTER · 1987 · $138,360

## Abstract

Red skeletal muscle exhibits the widest range of energy turnover of any
tissue.  It is therefore ideally suited to the study of controls of
glycolsis, aerobic ATP production, and the interaction of the two on the
regulation of ATP concentration.  Present understanding is largely based on
in vitro studies designed to solve pieces of the puzzle - i.e. feedback
control of phosphofructokinase, control of the redex shuttle in
mitochondria, etc.  The success of such work permits a synthesis which
explains how major pathways interact under physiologic conditions.

The principal obstacle to in vivo metabolic studies has been inability to
measure a complete set of metabolites including O2 per se simultaneously on
a single preparation under various planned conditions of energy turnover.
Metabolic and circulatory heterogeneities add further difficulties.  The
proposed experiments will be performed on a well-characterized dog gracilis
preparation containing red fibers exclusively.  All supply vessels are
preserved in the vascular isolation.  Intracellular PO2 and its regional
distribution is measured with a cryomicrospectroscopic method with spatial
resolution of 2-3 mitochondrial volumes.  All assays of tissue metabolites
are performed on the same samples used for O2 spectroscopy.  Muscles are
frozen in situ so all measurements are, in effect, simultaneous.  Succunic
dehydrogenase is used to scale for the oxidative capacity of each muscle.
Thus some concerns about spatial and temporal heterogeneities and biologic
variability can be overcome.

Intracellular pH, cytosolic phosphorylation and redox potentials,
glycolytic intermediates, O2, and parameters related to mitochondrial redox
will be determined and correlated with rates of O2 consumption and
glycolysis.  Measurements will be made at rest, during rest-work
transitions, during transitions between steady-state work rates during
simulated excercise in the steady state and, if time permits, in recovery.
Results will be used to evaluate current ideas of mitochondrial-cytosolic
interaction and pathway control in vivo.  The data will be used to develop
and evaluate quantitative mathematical models of integrated energy
metabolism in red muscle.

## Key facts

- **NIH application ID:** 3157490
- **Project number:** 5R01AR036154-02
- **Recipient organization:** UNIVERSITY OF ROCHESTER
- **SAM.gov UEI:** F27KDXZMF9Y8
- **Principal Investigator:** RICHARD J CONNETT
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $138,360
- **Award type:** 5
- **Project period:** 1986-04-01 → 1991-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3157490

## Citation

> US National Institutes of Health, RePORTER application 3157490, GLYCOLYSIS AND MITOCHONDRIA: INTERACTION IN VIVO (5R01AR036154-02). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3157490. Licensed CC0.

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