# MUSCLE ENERGY METABOLISM CARDIAC, SMOOTH AND SKELETAL

> **NIH NIH R01** · BRIGHAM AND WOMEN'S HOSPITAL · 1988 · $194,693

## Abstract

Muscle tissue offers the experimentor a range of metabolic capabilities
(fast-twitch glycolytic, slow-twitch oxidative, and cardiac highly
oxidative cells), and rates of energy utilization which may vary as much as
50 times during contractile activity compared to the basal rate.  With the
view that there is an overall unity in the mechanisms for integrating
cellular energetics in the different types of cells (however different they
may be in detail), we will use a comparative approach to measure
quantitatively mechanisms affecting muscle energetics.  What is the role of
intracellular pH in cellular energetics?  How well can we quantify the
unidirectional fluxes of the reaction catalyzed by creatine kinase in
different cell types?  We will test whether the creatine kinase reaction is
reversible during contraction as well as at rest, and develop quantitative
models to explain the results and to test concepts of metabolic
compartmentalization between myofibrils, cytosol and mitochondria.  I will
use slowly-metabolized creatine analogues to manipulate the system
further:  what is the basis for what appears to be the increased aerobic
metabolic adaptation?  How does the free energy available from the coupled
hydrolysis of ATP vary in various physiological states?  How significant is
the role of intracellular pH here?  In all these studies I will measure
non-invasively cellular phosphates by 31P-NMR, oxygen consumption by
conventional methods, and mitochondrial redox states by NADH fluorometry.
Finally, using the detailed information and concepts developed in the
isolated muscles and animal models, I will work to measure relevant
energetic parameters by 31P-NMR methods in human limbs, and to develop the
concept of a quantitative metabolic stress test.

## Key facts

- **NIH application ID:** 3157525
- **Project number:** 5R01AR036281-04
- **Recipient organization:** BRIGHAM AND WOMEN'S HOSPITAL
- **SAM.gov UEI:** QN6MS4VN7BD1
- **Principal Investigator:** Martin J Kushmerick
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $194,693
- **Award type:** 5
- **Project period:** 1985-02-01 → 1988-09-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3157525

## Citation

> US National Institutes of Health, RePORTER application 3157525, MUSCLE ENERGY METABOLISM CARDIAC, SMOOTH AND SKELETAL (5R01AR036281-04). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3157525. Licensed CC0.

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