# ALTERNATIVE SPLICING OF CONTRACTILE PROTEIN GENES

> **NIH NIH R01** · BOSTON CHILDREN'S HOSPITAL · 1993 · $226,767

## Abstract

DESCRIPTION:  (Adapted from the Investigator's abstract):  The overall goal
of the research proposed in this application continues to be the
identification and characterization of cis- and trans-acting factors
involved in the regulation of alternative splicing.  To this end, the
investigators will continue to use mini-gene constructs and particular
sequence elements from the contractile protein genes alpha-tropomyosin and
troponin T as a model system that will be analyzed using in vivo and in
vitro cell-free splicing system.  Three main specific aims will be pursued:
1.-To analyze and characterize the structure and mechanism of action of a
splicing factor, Polypyrimidine Binding Protein (PBP), which they have
recently purified and partially sequenced.  This factor plays an important
role in determining 3' splice strength and might be involved in the early
steps of splice site commitment.  2.-To further determine the binding site
of a negative splicing factor specific to smooth muscle cells.  To isolate,
purify, and characterize the mechanism of action of this factor that works
by blocking the default splicing pattern.  3.-Using a highly selective
genetic approach, to identify and characterize a positive splicing factor
specific to mature smooth muscle cells which determines the regulated
splicing pattern of the troponin T transcript.

It is expected that the cloning and characterization of these three
splicing factors will make a significant contribution to the understanding
of both constitutive and alternative models of splicing.

## Key facts

- **NIH application ID:** 3157785
- **Project number:** 5R01AR036865-08
- **Recipient organization:** BOSTON CHILDREN'S HOSPITAL
- **SAM.gov UEI:** Z1L9F1MM1RY3
- **Principal Investigator:** Bernardo Nadal Ginard
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $226,767
- **Award type:** 5
- **Project period:** 1986-01-01 → 1995-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3157785

## Citation

> US National Institutes of Health, RePORTER application 3157785, ALTERNATIVE SPLICING OF CONTRACTILE PROTEIN GENES (5R01AR036865-08). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3157785. Licensed CC0.

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