# BIOSYNTHESIS OF PROTEOGLYCANS

> **NIH NIH R01** · BRIGHAM AND WOMEN'S HOSPITAL · 1987 · $108,720

## Abstract

The objectives of this project are to investigate the biosynthesis of
glycosaminoglycans and proteoglycans with the eventual aim of determining
the overall mechanism of assembly.

Microsomal preparations from chick embryo cartilage and mast cells will be
utilized with sugar nucleotide precursors and PAPS to continue the
delineation of the pathway of chondroitin sulfate biosynthesis.  Microsomal
preparations from mouse mast cell tumors will be utilized similarly with
appropriate radioactively labeled sugar nucleotide precursors and PAPS to
continue the delineation of the pathway of heparin biosynthesis.
Microsomal preparations from the Englebreth-Holm-Swarm rat sarcoma and from
cultured bovine endothelial cells will be utilized with appropriate
radioactively labeled sugar nucleotide precursors and PAPS to delineate the
pathway of heparan sulfate biosynthesis.  In all of these cases the
structure of the proteoglycans at the site of synthesis will be examined.

Tissue cultures of endothelial cells producing heparant sulfate and of mast
cells producing both heparin and chondroitin sulfate will be utilized to
continue the investigation of the subcellular sites for proteoglycan
biosynthesis.  The processes of packaging and expert of the finished
compounds will be followed.

Radioactive precursors will be utilized to prelabel cartilage, tumors, and
cells before obtaining subcellular fractions for investigation of sites for
proteoglycan metabolism.

## Key facts

- **NIH application ID:** 3157847
- **Project number:** 5R01AR036984-03
- **Recipient organization:** BRIGHAM AND WOMEN'S HOSPITAL
- **SAM.gov UEI:** QN6MS4VN7BD1
- **Principal Investigator:** JEREMIAH E SILBERT
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $108,720
- **Award type:** 5
- **Project period:** 1985-09-01 → 1989-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3157847

## Citation

> US National Institutes of Health, RePORTER application 3157847, BIOSYNTHESIS OF PROTEOGLYCANS (5R01AR036984-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3157847. Licensed CC0.

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