# COLLAGEN STRUCTURE AND CROSSLINKING IN O.I. BONE

> **NIH NIH R01** · UNIVERSITY OF SOUTHERN CALIFORNIA · 1987 · $91,715

## Abstract

Osteogenesis imperfecta (OI) is a heterogenous group of
heritable connective tissue disorders usually associated with
bone fragility.  Biochemical studies suggest that molecular
defects of type I collagen are the root of the disorders.  A
wide variety of collagen abnormalities have been described but
only a limited number of well defined molecular defects have
been reported.  We plan to study collagen defects in this
disease by comparing the nature of the collagen structure,
fibrillogenesis and the process of crosslinking in normal and
effected bone and skin.  The main objective in the proposal is
to improve existing techniques for detecting minor defects in
Osteogenesis imperfecta collagen by:

1. 2-D CNBr peptide mapping and reverse phase HPLC
tryptic-mapping and finger printing of peptides recovered from
the 2-D maps.

2. Measuring the thermal stability and the renaturability of
procollagen synthesized by Osteogenesis imperfecta cells.

3. Examining the ability of Osteogenesis imperfecta collagen
can form fibers and crosslinks as efficiently as the normal
collagen.

Finally, we will adapt these findings to improve the protocol
for screening collagen defects in the prenatal diagnosis
procedure for Osteogenesis imperfecta.

## Key facts

- **NIH application ID:** 3157897
- **Project number:** 5R01AR037042-02
- **Recipient organization:** UNIVERSITY OF SOUTHERN CALIFORNIA
- **SAM.gov UEI:** G88KLJR3KYT5
- **Principal Investigator:** DAVID T CHEUNG
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $91,715
- **Award type:** 5
- **Project period:** 1986-08-01 → 1990-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3157897

## Citation

> US National Institutes of Health, RePORTER application 3157897, COLLAGEN STRUCTURE AND CROSSLINKING IN O.I. BONE (5R01AR037042-02). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3157897. Licensed CC0.

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