# GLYCOPROTEINS MEDIATING MYOBLAST RECOGNITION/FUSION

> **NIH NIH R01** · LANKENAU HOSPITAL · 1991 · $165,036

## Abstract

During the development of muscle, proliferating myogenic cells
differentiate and fuse to form multinucleate cells that synthesize proteins
necessary for the assembly of striated myofibrils.  One event previously
shown by the applicant to be critical to this multistep process is the
specific, Ca++-dependent adhesion of myoblasts prior to their fusion.  The
work proposed here aims to isolate and identify the cell surface molecules
mediating myoblast recognition using a combined biochemical and
immunological approach.  Recent work by the applicant indicates that the
cell surface adhesion molecules are glycoproteins.  Adhesion glycoproteins
are currently being purified from pectoral muscle explants and myoblast
cultures using anion exchange and lectin affinity chromomatrophy.  To date
our active fraction contains eight glycoproteins.  We are raising
monoclonal and polyclonal, monospecific antibodies against these
glycoproteins in an effort to isolate antibodies that inhibit
Ca++-dependent myoblast adhesion.  Monospecific, adhesion-perturbing
antibodies will be used to identify and localize the adhesion glycoproteins
and to study their expression during myogenesis.

## Key facts

- **NIH application ID:** 3158346
- **Project number:** 5R01AR037945-05
- **Recipient organization:** LANKENAU HOSPITAL
- **Principal Investigator:** KAREN A KNUDSEN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $165,036
- **Award type:** 5
- **Project period:** 1987-03-01 → 1992-02-29

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3158346

## Citation

> US National Institutes of Health, RePORTER application 3158346, GLYCOPROTEINS MEDIATING MYOBLAST RECOGNITION/FUSION (5R01AR037945-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3158346. Licensed CC0.

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