# PATHOPHYSIOLOGY OF MUSCLE IN VIVO AND IN VITRO NMR

> **NIH NIH R01** · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · 1987 · $72,465

## Abstract

Nuclear magnetic resonance (NMR) techniques are gaining increasing
acceptance in clinical practice and medical research.  With the development
of NMR scanners, it is now possible to obtain structural and functional
information of tissues in a living organism by non-invasive means.  The NMR
parameters such as the spin-lattice relaxation times (T1), spin-spin
relaxation times (T2), and chemical shifts of tissues are altered by
pathophysiologic events.  Spatial localization of these changes in the NMR
parameters provides the structural information in form of images, and
measurement of the magnitude of change gives an insight into the functional
state of tissue.  The NMR techniques will be used to study the in vivo and
in vitro pathophysiologic changes in the skeletal muscles of a
well-characterized animal model.  Experiments will be designed to test the
hypothesis that normal and pathophysiologic events alter the physical
properties of cellular water as measured by the NMR parameters.  Initial
studies will examine the relationship of T1 and T2 with the intrinsic
metabolic (oxidative or glycolytic) characteristics of several
predominantly fast and slow muscles.  The changes in the NMR
characteristics of these muscles during ontogenic development will be
determined.  Hypertrophy and atrophy will be induced in the muscles by
genetic and experimental manipulations and their specific effects on the
NMR parameters will be investigated.  Different techniques of measurements
will be used to distinguish structural and functional abnormalities in
muscles due to compensatory physiologic responses and pathologic
interventions.  The role of hydration in changing the NMR relaxation times
will be studied by controlled in vivo dehydration of muscles.
Pharmacologic intervention will be used to examine to what extent changes
in protein content and composition influence the NMR relaxation times of
muscles.  The contribution of protons from fat and water to the NMR
parameters of muscles will be determined in lines selected for high or low
fat.  Finally, we will examine the relationship between in vitro and in
vivo NMR measurement of muscles.  The results from all these experiments
will provide useful information for detection and monitoring the
progression of muscle diseases.

## Key facts

- **NIH application ID:** 3158774
- **Project number:** 5R01AR038741-02
- **Recipient organization:** UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
- **SAM.gov UEI:** ZUFBNVZ587D4
- **Principal Investigator:** LALITH K MISRA
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $72,465
- **Award type:** 5
- **Project period:** 1986-07-01 → 1988-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3158774

## Citation

> US National Institutes of Health, RePORTER application 3158774, PATHOPHYSIOLOGY OF MUSCLE IN VIVO AND IN VITRO NMR (5R01AR038741-02). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3158774. Licensed CC0.

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