# NMR STUDIES OF MAMMALIAN SKELETAL MUSCLE METABOLISM

> **NIH NIH R01** · MICHIGAN STATE UNIVERSITY · 1991 · $190,267

## Abstract

This   project will use NMR spectroscopy and other methods to examine the
role of changes in energetically important metabolites (e.g., PCr, ATP,
inorganic phosphate, lactic acid, pH) in the regulation of mammalian
skeletal muscle metabolism.  The major specific aim is to test competing
models of the control of respiration in intact muscle.  This will be
accomplished by examining the relationships between cytoplasmic phosphate
metabolites, mitochondrial NADH/NAD ratio (measured by surface
fluorescence) and respiration in cat fast (biceps) and slow-twitch
(soleus) muscles at rest and during and after stimulation.  Additional
studies will examine the effects of experimentally-imposed changes in pH,
PCr, ATP, and substrate supply on these relationships.  These experiments
will also examine the effects of imposed changes in pH and phosphate
metabolites on regulation of glycolysis and force development.
Additional experiments will examine whether there is functionally--
significant compartmentation of adenine nucleotides in muscle, test the
validity of 1H-NMR methods for measurement of lactic acid, and define the
relationships between pH and lactic acid during recovery after muscle
stimulation.  A key feature of these studies compared to other NMR
studies of muscle is the use of well-characterized perfused muscle
preparations, which enables experimental manipulations not possible in
muscles in situ.  The results will advance our basic understanding of
muscle metabolism, as well as provide essential background for the
rational design and interpretation of clinical NMR studies of muscle.

## Key facts

- **NIH application ID:** 3159024
- **Project number:** 2R01AR038972-04A1
- **Recipient organization:** MICHIGAN STATE UNIVERSITY
- **SAM.gov UEI:** R28EKN92ZTZ9
- **Principal Investigator:** Ronald A. Meyer
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $190,267
- **Award type:** 2
- **Project period:** 1987-07-01 → 1995-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3159024

## Citation

> US National Institutes of Health, RePORTER application 3159024, NMR STUDIES OF MAMMALIAN SKELETAL MUSCLE METABOLISM (2R01AR038972-04A1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3159024. Licensed CC0.

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