# PATHOGENIC ANTI-DNA ANTIBODIES--REGULATE HUMAN LUPUS

> **NIH NIH R01** · TUFTS MEDICAL CENTER · 1989 · $115,304

## Abstract

The major goal of this research project is to define the underlying
mechanisms of the human autoimmune disease Systemic Lupus Erythematosus
(SLE).  Pathogenic anti-DNA autoantibodies that cause glomerulonephritis
and other autoimmune lesions of SLE can be distinguished by their
immunochemical properties, charge and idiotypic profile.  The cellular and
humoral immunoregulatory imbalances in patients with SLE that specifically
induce the production of the select population of pathogenic autoantibodies
will be investigated in detail.  The specific set of T and B cells
responsible for pathogenic autoantibody production will be analyzed by
studying both subpopulations and clones.  T cell receptor variable region
genes expressed by the pathogenic autoantibody inducing T cells will be
analyzed and sequenced.  The major cross-reactive idiotype(s) shared
exclusively or preferentially by the pathogenic autoantibodies will be
identified.  The cellular and humoral immunoregulatory mechanisms
controlling the production of pathogenic autoantibodies bearing the
distinctive cross-reactive idiotype (CRI) will be defined by studying
lymphocyte subsets and clones.  Monoclonal human autoantibodies that share
the distinctive CRI of pathogenic anti-DNA autoantibodies will be derived
by hybridoma technology.  The immunochemistry, spectrotype and antigenic
specificity patterns of the CRI+ monoclonal autoantibodies will be studied.

The proposed studies will be important for designing specific immunotherapy
for rheumatic autoimmune diseases in humans and the experiments will also
yield basic information about immunoregulation in humans.

## Key facts

- **NIH application ID:** 3159131
- **Project number:** 1R01AR039157-01A1
- **Recipient organization:** TUFTS MEDICAL CENTER
- **SAM.gov UEI:** MY2ERHGDV956
- **Principal Investigator:** SYAMAL K DATTA
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $115,304
- **Award type:** 1
- **Project period:** 1989-06-15 → 1992-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3159131

## Citation

> US National Institutes of Health, RePORTER application 3159131, PATHOGENIC ANTI-DNA ANTIBODIES--REGULATE HUMAN LUPUS (1R01AR039157-01A1). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3159131. Licensed CC0.

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