# BONE CELL ANTIGENS RELATED TO PTH RECEPTORS

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA RIVERSIDE · 1990 · $166,800

## Abstract

The goal of this research is to characterize cell membrane antigens
recognized by a panel of monoclonal antibodies which bind specifically to
mouse osteoblasts, especially those antigens which are associated with the
function of the parathyroid hormone (PTH) receptor.  Antibodies which bind
specifically to osteoblasts, as determined by histology and flow cytometry,
will be surveyed.  The ability of antibodies to influence PTH receptor
activities will be examined in cultured mouse bone and isolated populations
of mouse bone cells.  Activities to be examined include binding of PTH and
labeled PTH analogs, activation of adenylate cyclase, suppression of
alkaline phosphatase and induction of bone resorption.  Antigens associated
with the osteoblast PTH receptor will be isolated by immunoaffinity
techniques and further characterized biochemically.  Antibodies which these
experiments indicate are specific for components of the PTH receptor system
will be used to screen recombinant expression libraries for nucleotide
sequences coding for the relevant antigens, with the objective of
determining the complete cDNA sequence of osteoblast PTH receptors.  Both
the antibodies and the isolated nucleic acid probes will be used to
evaluate expression of the PTH receptor gene(s) in osteoblasts and related
cells.  These investigations will provide detailed information about the
unique characteristics of bone cell surfaces and the molecular processes
involved in PTH action.  This knowledge will allow a better understanding
of changes in bone cell function in a number of bone-related diseases
including osteoporosis, rheumatoid arthritis, pseudohypoparathyroidism and
neoplastic bone destroying conditions.

## Key facts

- **NIH application ID:** 3159233
- **Project number:** 5R01AR039241-02
- **Recipient organization:** UNIVERSITY OF CALIFORNIA RIVERSIDE
- **SAM.gov UEI:** MR5QC5FCAVH5
- **Principal Investigator:** RICHARD A LUBEN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $166,800
- **Award type:** 5
- **Project period:** 1989-03-01 → 1992-02-29

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3159233

## Citation

> US National Institutes of Health, RePORTER application 3159233, BONE CELL ANTIGENS RELATED TO PTH RECEPTORS (5R01AR039241-02). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3159233. Licensed CC0.

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