# T CELLS AND DISEASE

> **NIH NIH R01** · DANA-FARBER CANCER INST · 1990 · $162,758

## Abstract

We propose to study the clonal heterogeneity of T lymphocytes in
autoimmune diseases, particularly in rheumatoid arthritis.  This
will be carried out through an analysis of the T cell receptors
(TCR).  Framework monoclonal antibodies (mAb) against the TCR delta
chain (beta F1) and against the TCR beta chain (anti-TCR delta 1)
will be used to study the TCR alpha beta and TCR gamma delta
proteins from rheumatoid arthritis T cells.  The T cells will
include the activated T cells from peripheral blood, as well as T
cells from synovial fluid and synovial tissue.  TCR protein will
be characterized by two dimensional gel analyses.  TCR gene
rearrangements will be examined in Southern blotting analyses.  TCR
protein will then be isolated for frequently immunization of mice
in the production of mAb that may recognize frequently used or
cross-reactive TCR gene segment encoded determinants.
Complementary used or cross-reactive TCR gene segment encoded
determinants.  DNA clones corresponding to these gene segments will
be cloned and sequenced.  Analysis on a more limited basis will be
carried out in type 1 diabetes and systemic lupus erythematosus.
The framework mAb against the TCR will also be evaluated as
diagnostic tools for distinguishing malignant versus benign T-
lineage proliferations, based on the discordant expression of CD3
and TCR proteins in neoplasia.  All analyses performed will include
both receptors, TCR alpha beta and TCR gamma delta.  We hope that
the proposed studies will help us to understand the role of T cells
in autoimmune diseases and that T cell receptors may prove useful
in the diagnosis of T-lineage neoplasms.

## Key facts

- **NIH application ID:** 3159736
- **Project number:** 5R01AR039582-03
- **Recipient organization:** DANA-FARBER CANCER INST
- **SAM.gov UEI:** DPMGH9MG1X67
- **Principal Investigator:** Michael B. Brenner
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $162,758
- **Award type:** 5
- **Project period:** 1988-07-01 → 1993-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3159736

## Citation

> US National Institutes of Health, RePORTER application 3159736, T CELLS AND DISEASE (5R01AR039582-03). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3159736. Licensed CC0.

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