# BIOENERGETIC AND CA2+ REGULATION OF MUSCLE IN FATIGUE

> **NIH NIH R01** · WASHINGTON STATE UNIVERSITY · 1991 · $316,526

## Abstract

Experiments will be conducted to determine the effects of exhaustive
exercise on mitochondrial and sarcoplasmic reticulum function and structure
of skeletal muscle. Thoroughbred horses and rate will be exposed to a
variety of exercise durations and intensities until fatigue. Samples will
be collected from trained and non-trained animals in association with the
exercise. Horses will be used for studies where the exercise intensity is
varied as a function of the individual's  maximal oxygen uptake. This will
be done on a high speed equine treadmill which permits regulation of the
intensity of the exercise and metabolic response of the animals. They will
be exercised at submaximal, maximal supramaximal exercise intensities,
Horses will be trained for 12 weeks exercising up to 30 miles per week on a
half mile training track. Rats will be used to study the effects of long
term exercise on the different types of muscle fibers. These animals are
ideal for such studies as they have distinctive muscle regions composed
almost exclusively of a single fiber type. Rats will be endurance trained
for up to 26 weeks with a strenuous program involving running twice per day
for up to 3 hr per day. In addition to the studies conducted to determine
the mechanisms by which the exercise may induce changes, the potential for
training to ameliorate these effects will also be assessed. Studies will
involve evaluation of mitochondrial respiratory rate and capacity, lipid
oxidation and oxidation of sulfhydryl groups in the sarcoplasmic reticulum
changes in phosphoprotein formation and ATP binding in the sarcoplasmic
ATPase, mitochondrial hexokinase binding, alterations in the activities of
enzymes reducing oxygen free radical formation, and changes in the
concentrations of tissue metabolites that may influence mitochondrial
respiratory capacities. The time course for recovery of exercise-induced
effects will be determined.

## Key facts

- **NIH application ID:** 3159745
- **Project number:** 5R01AR039583-03
- **Recipient organization:** WASHINGTON STATE UNIVERSITY
- **SAM.gov UEI:** XRJSGX384TD6
- **Principal Investigator:** KENNETH B CAMPBELL
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $316,526
- **Award type:** 5
- **Project period:** 1989-09-20 → 1993-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3159745

## Citation

> US National Institutes of Health, RePORTER application 3159745, BIOENERGETIC AND CA2+ REGULATION OF MUSCLE IN FATIGUE (5R01AR039583-03). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3159745. Licensed CC0.

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