# MYOSIN ROD SEQUENCES AND THEIR ROLE IN FILAMENT ASSEMBLY

> **NIH NIH R01** · UNIVERSITY OF PENNSYLVANIA · 1991 · $220,747

## Abstract

The overall aim of this project is to understand the mechanism by which
myosin molecules self-assemble to form myosin filaments. To achieve this
aim recombinant DNA technology will be used. Starting with fragments of the
myosin molecule which have characteristic assembly properties we will
define the human embryonic myosin heavy chain (HEMHC) gene segment
corresponding to the fragment. Starting with synthetic oligonucleotides
flanking the region of interest we will amplify the gene segment using the
polymerase chain reaction. This segment will be expressed in E. coli to
verify that it retains the assembly characteristics. The gene segment will
then be modified by shortening or elongating to express different size
fragments. In this way the changes in the assembly characteristics related
to different portions of the myosin molecule will be defined. From the
amino acid sequence, characteristic properties of the sequence will be
related to the assembly characteristics. Using this information a mechanism
for the assembly of myosin should evolve.

## Key facts

- **NIH application ID:** 3159762
- **Project number:** 1R01AR039590-01A3
- **Recipient organization:** UNIVERSITY OF PENNSYLVANIA
- **SAM.gov UEI:** GM1XX56LEP58
- **Principal Investigator:** FRANK A PEPE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $220,747
- **Award type:** 1
- **Project period:** 1991-01-01 → 1994-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3159762

## Citation

> US National Institutes of Health, RePORTER application 3159762, MYOSIN ROD SEQUENCES AND THEIR ROLE IN FILAMENT ASSEMBLY (1R01AR039590-01A3). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3159762. Licensed CC0.

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