# MAREK'S DISEASE VIRUS:  TRANSFORMATION AND ONCOGENESIS

> **NIH NIH R01** · TAMPA BAY RESEARCH INSTITUTE · 1987 · $145,665

## Abstract

This proposal contains specific features of the studies on the oncogenicity
of Marek's disease virus (MDV).  In specific aims I, II and III, we plan to
study the detailed aspect of D-H region which is uniquely expanded in
nonpathogenic strains of MDV and which may contain a possible tumor
inducing gene of MDV.  We will obtain more biological evidence that D-H
region may be needed for tumor induction and/or transformation.  This will
be done by proposed studies such as co-transfection of nonpathogenic viral
DNA with D-H fragment to recover pathogenic viral strain, transformation or
immortalization of cells by D-H transfection, and determination of
tumorgenicity in chicken for viruses with normal D-H and expanded D-H,
plaque purified from a weakly oncogenic virus strain.  MDV-induced
lymphoblastic cell DNA will be tested for NIH 3T3 cell transformation to
examine whether chicken cell DNA sequence may be present for inducing HIH
3T3 cell transformation.  Structure and transcriptional patterns of D-H
region will be studied.  D-H gene products will be synthesized by mammalian
vector or bacterial plasmid and characterized.  Other unique structural
features of MDV DNA will also be studied.  Other viral genes actively
transcribed in transformed cells and tumors will be identified.  Finally,
DNA of herpesvirus turkey (HVT), which is used as a vaccine for prevention
of MD, will be physically mapped by restriction enzymes and colinearity of
viral DNA between MDV and HVT will be determined.  Cross-reactive antigens
between MDV and HVT will be assigned to the maps of both virus by in vitro
transplantation method.  This study will be important for the future
genetic study of MDV and HVT.

## Key facts

- **NIH application ID:** 3170048
- **Project number:** 5R01CA031949-07
- **Recipient organization:** TAMPA BAY RESEARCH INSTITUTE
- **Principal Investigator:** MEIHAN NONOYAMA
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $145,665
- **Award type:** 5
- **Project period:** 1981-08-01 → 1988-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170048

## Citation

> US National Institutes of Health, RePORTER application 3170048, MAREK'S DISEASE VIRUS:  TRANSFORMATION AND ONCOGENESIS (5R01CA031949-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170048. Licensed CC0.

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