# MAREK'S DISEASE VIRUS--TRANSFORMATION & ONCOGENESIS

> **NIH NIH R01** · TAMPA BAY RESEARCH INSTITUTE · 1992 · $189,246

## Abstract

After the restriction enzyme mapping of the MDV genome, we and others found
an amplification of a 132 bp repeat in IRL and TRL, which caused loss of
tumorgenicity of MDV.  In our recent study, the loss of tumorgenicity was
found to be due to the disruption of a 1.8 kb RNA gene as well as a 3.8 kb
RNA gene.  Exon-I is mapped to the 5'-end of the amplification and exon-II
is mapped to the 3'-downstream region of the amplification.  In this study
we propose to further investigate the nature of the 1.8 kb RNA gene as well
as the 3.8 kb RNA gene.  We plan to make cDNA libraries of oncogenic and
non-oncogenic MDV infected cell RNA and precisely map the 1.8 kb and 3.8 kb
RNA be raising monospecific antibody to these genes.  Once we determine the
open reading frames of these genes and raise monospecific antibodies to
these gene products, we will reconstitute oncogenic MDV by insertion of
these genes back into the non-oncogenic MDV genome at the region of the TK
gene or at the truncated RNA region.  This will give direct evidence that
these genes of MDV (1.8 kb and/or 3.8 kb RNA) are indispensable for tumor
induction in chicken.  Furthermore, eukaryotic expression vectors made by
inserting the cDNA or the open reding frame of these genes into pZIPneo or
3'-downstream of RSV promoter will be used to determine whether H gene can
transform NIH 3T3 cells or CEF.  These proposed studies will further
clarify the role of these genes in inducing tumors in chicken.

## Key facts

- **NIH application ID:** 3170049
- **Project number:** 5R01CA031949-09
- **Recipient organization:** TAMPA BAY RESEARCH INSTITUTE
- **Principal Investigator:** MEIHAN NONOYAMA
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1992
- **Award amount:** $189,246
- **Award type:** 5
- **Project period:** 1981-08-01 → 1995-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170049

## Citation

> US National Institutes of Health, RePORTER application 3170049, MAREK'S DISEASE VIRUS--TRANSFORMATION & ONCOGENESIS (5R01CA031949-09). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170049. Licensed CC0.

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