# HUMORAL CONTROL OF LEUKEMIC BLAST PROLIFERATION

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA, SAN DIEGO · 1990 · $124,175

## Abstract

This proposal is a continuation of studies examining soluble factors
controlling or modulating human, myeloid leukemia cell growth.  In the
past, the actions of colony-stimulating factor (CSF) on normal and acute
non-lymphocytic leukemia cell (ANNL) growth were compared.  Monoclonal
antibodies to transferrin (Tf) receptors were employed to examine roles of
Tf, Fe, and Tf receptors in myeloid leukemia cell growth.  In this
proposal, specialized serum-free, Tf-free, and protein-free cultures of
myeloid leukemia cell lines will be used to assess cytokinetic effects of
Tf, Fe, insulin, and somatomedins on ANNL cell growth.  These effects will
be correlated with effects on cellular oncogene(c-onc) gene and Tf receptor
expression.  The c-onc gene responses to these nutritionally-related
molecules wil be contrasted to cell line responses to CSF.  For the latter
studies, HL60 and KG-1 cell lines induced to differentiate and to respond
to CSF will be used.  Cytokinetic responses to CSF will also be assessed,
and coupling of CSF response to differentiation-related, cell surface
antigens assssed.  CSF effects on self-renewal will be examined using
colony and continuous cultures of fresh ANLL cells and leukemia cell lines
treated with differentiation-inducers and CSF.  Anti-polypeptide, CSF
antibodies will be prepared and tested for effects on normal cells and
differentiation-induced cell lines and ANLL cells.  To continue studies of
Tf, Fe and Tf receptors in ANLL cell growth, regulation of Tf receptor gene
expression will be studied in Fe-loaded and serum-free cultured cells, ANLL
cells induced to differentiate, cells cultured with anti-Tf receptor
antibodies, and cells in transition from resting to proliferating states.
A TfR cDNA will be used for this purpose and Tf receptor transcript levels
correlated with rates of specific protein and mRNA synthesis.  Studies of
Tf's role in ANLL growth will also examine mechanisms by which Tf enhances
CSF release from normal cells and increases ANLL cell growth.  For these
studies, effects of internalized ligands such as Tf, alpha2-macroglobulin,
LDL, and Fc receptor bound immunoglobulin on CSF generation and Tf receptor
turnover will be determined.  The Tf receptor positive adherent blood and
marrow cells will be characterized using monoclonal antibodies, and effects
of Tf compared to Ca ionophore.  Finally, Tf effects on long-term normal
and ANLL marrow growth will be assessed.

## Key facts

- **NIH application ID:** 3170122
- **Project number:** 5R01CA032094-08
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN DIEGO
- **SAM.gov UEI:** UYTTZT6G9DT1
- **Principal Investigator:** RAYMOND M TAETLE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $124,175
- **Award type:** 5
- **Project period:** 1982-06-01 → 1990-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170122

## Citation

> US National Institutes of Health, RePORTER application 3170122, HUMORAL CONTROL OF LEUKEMIC BLAST PROLIFERATION (5R01CA032094-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170122. Licensed CC0.

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