# PROTEIN PATTERNS IN LEUKEMIC CELLS

> **NIH NIH R01** · UNIVERSITY OF MICHIGAN AT ANN ARBOR · 1990 · $205,821

## Abstract

This renewal application has two related objectives.  One is the
detailed analysis by two-dimensional polyacrylamide gel
electrophoresis (2-D  PAGE) of lymphoid cells for the purpose of
detecting polypeptides that are abnormally expressed in acute
lymphoblastic leukemia (ALL).  The analysis will benefit from the
increased resolution of 2-D PAGE we have achieved, the development
of computer programs for polypeptide spot matching and from the
availability of a lymphoid protein database that we have
established and that we propose to expand.  The second objective
consists of detailed investigations of a selected group of
polypeptides of interest.   We propose to focus initially on four
polypeptides detected in ALL in the initial grant cycle,
specifically: LT4 (T cell marker found in abundance in T ALL), L4
(common ALL marker), L3 (a polypeptide that is deficient in ALL
subtypes associated with poor prognosis), and p18 (a polypeptide
present in relatively high amount in ALL but not in other lymphoid
proliferating cells examined).  An immobilized pH gradient based
approach, developed in this laboratory, will be relied upon to
generate preparative amounts of polypeptides for
sequencing and for monoclonal antibody production.  Antibodies will
be utilized to determine the occurrence of the corresponding
polypeptides in single cells and for screening of samples using a
simple one-dimensional electrophoresis technique.  These studies
are expected to result in a better understanding of the extent to
which the pathogenetic process in leukemia is associated with
abnormalities in a major constituent of cells through which
function is mediated, namely the proteins.

## Key facts

- **NIH application ID:** 3170147
- **Project number:** 5R01CA032146-06
- **Recipient organization:** UNIVERSITY OF MICHIGAN AT ANN ARBOR
- **SAM.gov UEI:** GNJ7BBP73WE9
- **Principal Investigator:** SAMIR M HANASH
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $205,821
- **Award type:** 5
- **Project period:** 1983-02-01 → 1991-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170147

## Citation

> US National Institutes of Health, RePORTER application 3170147, PROTEIN PATTERNS IN LEUKEMIC CELLS (5R01CA032146-06). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3170147. Licensed CC0.

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