# FUNCTIONAL CLONES IN AUTOIMMUNE DISEASE

> **NIH NIH R01** · TUFTS UNIVERSITY BOSTON · 1988 · $181,777

## Abstract

The objective of this application is to assess whether or not
autoimmune disease in MRL mice is the result of T cell
recognition of aberrant (non H-2) kappa MHC antigens which are
expressed during active disease.

1.  Aim: (NEW) Subpopulations of lymphoid and non-lymphoid cells
derived from MRL/1pr or MRL/++ mice of different ages will be
evaluated using an extensive panel of monoclonal antibodies to
Class I and Class II antigens.  Any subsets of cells which
specifically stain with non H-2 kappa antibodies will be sorted and
reanalyzed by FACS.  They will additionally be evaluated by
functional analyses in vitro, and by 2D gel electrophoresis.
2.  Aim: (REVISED) Heterogeneous T cells, panned or sorted
L3T4+ and Ly2+ cells derived from different aged MRL mice will
be evaluated for their auto and alloreactivity in vitro using a
panel of stimulator cells.
3.  Aim: (REVISED) Heterogeneous T cells, panned or sorted
L3T4+ cells and T cell clones from different aged MRL mice will
be evaluated for their autoreactivity to vascular smooth muscle
cells derived from MRL mice with vasculitis.
4.  Aim (REVISED) Cell lines and clones will be recovered from
MuLV-M infected MRL mice.  Their phenotype and genotype will
be determined and the capacity to activate MRL T cells in vitro
will be explored.
5.  Aim: (NEW) The basis of T cell anergy in MRL mice will be
addressed.  Macrophages from MRL/1pr or MRL/++ with
autoimmune disease will be cocultured with T cells and T cell
subsets, and the (possible) induction of suppression in the L3T4+ T
cell subset evaluated.

Methodology: Fluorescence Activated Cell Sorter Analysis and
Sorting, mixed lymphocyte cultures, chromium release assays,
pepsin digestion of monoclonal antibodies, the culture of vascular
smooth muscle cells, panning of T cell subpopulations, retrovirus-
infection of MRL/1pr and MRL/++ mice; cloning of retrovirus-
infected cells.

## Key facts

- **NIH application ID:** 3170154
- **Project number:** 2R01CA032155-06A2
- **Recipient organization:** TUFTS UNIVERSITY BOSTON
- **SAM.gov UEI:** C1F5LNUF7W86
- **Principal Investigator:** CAROL LOUISE REINISCH
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $181,777
- **Award type:** 2
- **Project period:** 1981-07-01 → 1991-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170154

## Citation

> US National Institutes of Health, RePORTER application 3170154, FUNCTIONAL CLONES IN AUTOIMMUNE DISEASE (2R01CA032155-06A2). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170154. Licensed CC0.

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