# CHARACTERIZATION OF THE ALV-INDUCED TRANSFORMED FOLLICLE

> **NIH NIH R01** · WEST VIRGINIA UNIVERSITY · 1990 · $132,843

## Abstract

Avian leukosis virus induces a bursal dependent B cell lymphoma in the
chicken.  A rare histopathological lesion, the transformed follicle,
appears in the bursa shortly after infection.  It has been proposed that
this lesion is a preneoplastic stage required for the development of the
lymphoma.  The long range goal of this project is to define the biochemical
characteristics that distinguish the preneoplastic cell from the normal
cell.  The preneoplastic cell represents an intermediate stage of
neoplastic development that deserved considerable study.  Analysis of the
transformed follicle should provide information fundamental to an
appreciation of the biochemical and physiological conditions that increase
the propensity of a cell to develop into a tumor.

The cells within the ALV-induced transformed follicle will be characterized
by in situ hybridization and immunochemical analysis in order to identify
the presence of specific mRNAs and proteins.  A recombinant retroviral
vector will be used to evaluate the hypothesis that the expression of c-myc
results in the formation of the preneoplastic event.  cDNA hybridization
probes specific for the transformed follicle and the tumor will be
developed by subtractive hybridization in order to identify clones within a
cDNA library.  These genes, expressed preferentially in preneoplastic or
neoplastic tissue, will be characterized and the sequences encoding the
protein inserted into retroviral vectors.  Virus prepared from these
vectors will be used in an in vivo assay to assess the functional
significance of the expression of these genes in establishing the
preneoplastic state.

## Key facts

- **NIH application ID:** 3170272
- **Project number:** 7R01CA032295-08
- **Recipient organization:** WEST VIRGINIA UNIVERSITY
- **SAM.gov UEI:** M7PNRH24BBM8
- **Principal Investigator:** ERIC H. HUMPHRIES
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $132,843
- **Award type:** 7
- **Project period:** 1983-06-01 → 1991-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170272

## Citation

> US National Institutes of Health, RePORTER application 3170272, CHARACTERIZATION OF THE ALV-INDUCED TRANSFORMED FOLLICLE (7R01CA032295-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170272. Licensed CC0.

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