# ROLE OF PP60C-SRC HOMOLOG OF THE RSV ONCOGENIC PROTEIN

> **NIH NIH R01** · PENNSYLVANIA STATE UNIVERSITY, THE · 1988 · $144,656

## Abstract

A large-body of experimental data suggests that activated cellular
oncogenes may be responsible for at least some human neoplasias.
The c-oncs whose proteins have tyrosine kinase activity comprise
a subclass whose mechanisms of transformation are probably closely
related.  src is the best studied representative of this class.
We have shown that the kinase and transforming activities of its
normal protein product, pp60c-src, can be controlled by
phosphorylation at tyrosines 416 and 527 and have found evidence
suggesting that these phosphorylations are transiently altered in
overexpressed pp60c-src in vivo.  In addition, we have shown that
pp60c-src has enhanced specific kinase activity and is specifically
phosphorylated at an amino region threonine and (possibly) at
additional serine residues during mitosis.

Using recombinant DNA, gene transfer and cell synchronization
techniques with established cell lines, we will explore the
implication that pp60c-src plays a role in progression through
mitosis and the possibility that it is regulated in a cell cycle
dependent manner in other phases of the cycle as well.  In related
experiments, we will extend our studies of the regulatory roles of
tyrosine 416 and 527 phosphorylation and amino region
modifications.  In collaboration with other laboratories we will
use antibody microinjection and antisense techniques to see if c-
src activities are required for progression through the cell cycle
and/or response to growth factors and will study the effects of
expression of pp60c-src and pp60c-src phosphorylation site mutants
in transgenic mice.

These studies are part of our long term goal to trace the molecular
pathways of transformation by an oncogene as a model for
carcinogenesis.

## Key facts

- **NIH application ID:** 3170284
- **Project number:** 2R01CA032317-07
- **Recipient organization:** PENNSYLVANIA STATE UNIVERSITY, THE
- **SAM.gov UEI:** NPM2J7MSCF61
- **Principal Investigator:** DAVID I. SHALLOWAY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $144,656
- **Award type:** 2
- **Project period:** 1982-04-01 → 1993-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170284

## Citation

> US National Institutes of Health, RePORTER application 3170284, ROLE OF PP60C-SRC HOMOLOG OF THE RSV ONCOGENIC PROTEIN (2R01CA032317-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170284. Licensed CC0.

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