# ROLE OF PP60C-SRC HOMOLOG OF THE RSV ONCOGENIC PROTEIN

> **NIH NIH R01** · PENNSYLVANIA STATE UNIVERSITY, THE · 1987 · $141,585

## Abstract

We propose to continue our investigation of the role of pp60c-src, the
cellular homolog of pp60v-src, the transforming protein of Rous sarcoma
virus, in cellular growth control to determine what functional differences
between the two proteins are responsible for v-src induced neoplastic
transformation.  We will compare pp60c-src, pp60v-src, and
pp60recombinant-src (pp60r-src) activities in vivo using the c-, v- and
r-src expression plasmids and overexpressor cell lines that we have created
and will use immunopurified pp60src from these overexpressor cell lines for
in vitro studies.

We will determine if the low-frequency focus formation induced by extremely
efficient c-src overexpression plasmids is due to mutation or represents an
activity of authentic pp60c-src.  We will see if the defect in pp60c-src
transforming activity can be activated or complemented in tissue culture by
other transforming proteins, tumor promoters or hormones and will
investigate the biological effects of pp60c-src overexpression in
non-immortalized cells.

We will investigate the dose-response behaviors of cells to pp60c-src,
pp60v-src and pp60r-src overexpression to compare the behaviors of these
proteins in vivo.  We will complete the characterization of the activities
of c-src - v-src chimeric genes and will determine what biochemical
differences are associated with the different biological phenotypes induced
by these genes.

We intend to identify specific functional difference(s) between pp60c-src
and pp60v-src which are responsible for their different transforming
activities by comparing their in vitro and in vivo phosphorylating
activities.  In particular, we will investigate the significance of
pp60c-src phosphorylation of hexokinase.

## Key facts

- **NIH application ID:** 3170288
- **Project number:** 5R01CA032317-06
- **Recipient organization:** PENNSYLVANIA STATE UNIVERSITY, THE
- **SAM.gov UEI:** NPM2J7MSCF61
- **Principal Investigator:** DAVID I. SHALLOWAY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $141,585
- **Award type:** 5
- **Project period:** 1982-04-01 → 1988-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170288

## Citation

> US National Institutes of Health, RePORTER application 3170288, ROLE OF PP60C-SRC HOMOLOG OF THE RSV ONCOGENIC PROTEIN (5R01CA032317-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170288. Licensed CC0.

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