# ANTIGENIC ANALYSIS OF HEMATOPOIESIS

> **NIH NIH R01** · JOHNS HOPKINS UNIVERSITY · 1987 · $219,075

## Abstract

More successful therapy of hematologic disorders including
malignancies requires increased information concerning the
processes involved in normal hematopoietic differentiation.  A
major long-term goal of this work is to understand the critical
early events in normal human hematopoiesis which occur as a cell
"decides" between self-renewal and lineage committment and as a
cell matures along a lineage pathway.

Direct experiments in this area require purified hematopoietic
progenitor cells, and therefore would be enabled by a comprehensive
battery of monoclonal antibodies recognizing antigens expressed
specifically on early hematopoietic cell types.  The epitope
detected by the anti-My-10 monoclonal antibody is expressed
specifically on human marrow blast cells, including essentially all
measured hematopoietic progenitor cells.

In part I of this project, monospecific rabbit antisera will be
prepared against the purified My-10 glycoprotein, in order to
determine whether expression of the entire My-10 glycoprotein is
restricted to early hematopoietic developmental stages.  Additional
monoclonal antibodies against the My-10 glycoprotein will also be
produced, to allow improved labelling and isolation of My-10-
positive marrow progenitor cells.  Methods for affinity
purification of My-10-positive cells will be tested, as feasibility
studies prior to a clinical trial of the use of purified My-10-
positive "stem cells" for clinical bone marrow transplantation with
a reduced risk of Graft versus Host Disease.

Novel specific anti-human hematopoietic progenitor cell monoclonal
antibodies will also be generated in this project and will be used
in analysis and isolation of hematopoietic progenitor cells.

The second major specific goal of this project is the use of these
and other available monoclonal antibodies, with flow cytometry, to
construct detailed "maps" of normal hematopoietic cellular
development.  These maps will be used to investigate the cellular
events in normal versus abnormal myeloid cell.

## Key facts

- **NIH application ID:** 3170296
- **Project number:** 2R01CA032318-04A2
- **Recipient organization:** JOHNS HOPKINS UNIVERSITY
- **SAM.gov UEI:** FTMTDMBR29C7
- **Principal Investigator:** CURT I CIVIN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $219,075
- **Award type:** 2
- **Project period:** 1982-05-01 → 1990-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170296

## Citation

> US National Institutes of Health, RePORTER application 3170296, ANTIGENIC ANALYSIS OF HEMATOPOIESIS (2R01CA032318-04A2). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170296. Licensed CC0.

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