# STUDIES OF INTEGRATED MURINE LEUKEMIA VIRUS DNA

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA-IRVINE · 1989 · $133,026

## Abstract

A molecular study of chromatin containing integrated murine leukemia virus
DNA will be performed.  These experiments will provide model systems to
investigate 1) chromatin configuration around gene regulatory regions and
2) chromosomal position effect.

Moloney murine leukemia viruses (M-MuLV's) containing altered LTR's will be
generated by recombinant DNA manipulations.  DNA sequences conferring
hormone or metal responsiveness will be inserted, as will heterologous
transcriptional enhancersers.  M-MuLV's carrying the modified LTR's will be
recovered by transfection, and tested for hormone or metal responsiveness
or change in host range.  The chromatin structure of the altered proviruses
will be investigated, and the appearance of new DNAse I hypersensitive
sites or bound proteins will be correlated with the biological activity.
Promoter activity of the altered LTR's will also be assessed by transient
expression assays.

Chromatin structure of transcribed and non-transcribed M-MuLV proviruses
from a cell line chronically infected with M-MuLV will be investigated.
Recombinant clones of individual M-MuLV proviruses will be used to identify
these proviruses.  Nuclear run-off transcription will be employed.  The
chromatin state of the unoccupied host site as well as the proviral
integration will be investigated by DNase I digestion.

Cellular proteins which associate with the M-MuLV LTR will be identified by
procedures which reveal specific DNA-binding proteins.  The location of
protein binding in the LTR will be mapped, and LTR's which contain
deletions and insertions will be studied.  Extracts from different cell
types will be tested.  Correlation with the in vivo chromatin studies may
reveal specific proteins responsible for particular features of chromatin
structure.

## Key facts

- **NIH application ID:** 3170370
- **Project number:** 5R01CA032454-09
- **Recipient organization:** UNIVERSITY OF CALIFORNIA-IRVINE
- **SAM.gov UEI:** MJC5FCYQTPE6
- **Principal Investigator:** HUNG Y. FAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $133,026
- **Award type:** 5
- **Project period:** 1981-09-01 → 1991-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170370

## Citation

> US National Institutes of Health, RePORTER application 3170370, STUDIES OF INTEGRATED MURINE LEUKEMIA VIRUS DNA (5R01CA032454-09). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170370. Licensed CC0.

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