# EXPRESSION AND PATHOGENESIS OF MURINE LEUKEMIA VIRUS

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA-IRVINE · 1991 · $186,594

## Abstract

A study of the expression and pathogenesis of Moloney murine
leukemia virus (M-MuLV) is proposed.  Experiments on expression
center on tissue-specificity.  In particular, elements within the
long terminal repeat (LTR) will be investigated.  We will insert or
substiture sequences form heterologous viral and cellular genes
into the M-MuLV by recombinant cloning techniques.  The
sequences will include enhancer, promoter, and promoter-
proximal elements.  The altered LTR's will be tested for
expression in a variety of differentiated cell types.  In addition,
the altered LTR's will be reconstituted into replication-
component M-MuLV and tested for tissue-specific infection in
vitro and in vivo.  These experiments may provide important
information on tissue-specific transcription, and they may also be
important in the design of retroviral vectors.

Experiments on M-MuLV pathogenesis  take advantage of the fact
that several M-MuLV's with altered LTR's show altered
pathogenic potential.  This ranges from non-pathogenic M-MuLV's
(Mo+PyF101 M-MuLV) to M-MuLV's with altered disease spectrum
(PyF101+Mo M-MuLV).  These viruses provide a powerful tool for
investigating the nature of leukemic and preleukemic changes
induced by M-MuLV.  Hematopoietic tissue from preleukemic
mice inoculated with wild-type M-MuLV will be characterized for
preleukemic changes.  Hyperplasia will be documented and
characterized by flow cytometry and biological stem cell assays.
The viral status of preleukemic cells will also be investigated.
Mice inoculated with the variant viruses will also be examined for
preleukemic charges.  The variant viruses may identify both
critical and irrelevant preleukemic changes.  End-stage tumors
induced by wild-type and variant M-MuLV's will also be studied.
The cell types of the tumors will be determined, and the nature of
the viruses in the tumors will be determined.  The tumors will also
be examined for proviral insertion in the vicinity of cellular
proto-oncogenes.  These studies may identify critical steps in M-
MuLV induced leukemogenesis.

## Key facts

- **NIH application ID:** 3170378
- **Project number:** 5R01CA032455-11
- **Recipient organization:** UNIVERSITY OF CALIFORNIA-IRVINE
- **SAM.gov UEI:** MJC5FCYQTPE6
- **Principal Investigator:** HUNG Y. FAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $186,594
- **Award type:** 5
- **Project period:** 1982-02-01 → 1992-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170378

## Citation

> US National Institutes of Health, RePORTER application 3170378, EXPRESSION AND PATHOGENESIS OF MURINE LEUKEMIA VIRUS (5R01CA032455-11). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170378. Licensed CC0.

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