# HEMOPOIETIC STEM CELL DIFFERENTIATION TO MACROPHAGES

> **NIH NIH R01** · ALBERT EINSTEIN COLLEGE OF MEDICINE · 1989 · $261,437

## Abstract

The overall aim of this project is to investigate the mechanisms by which
pluripotent hemopoietic stem cells give rise to macrophages to characterize
the growth factors involved in this process. Three factors implicated in
this pathway have been studied.  They are the colony stimulating factor-1
(CSF-1), hemopoietin-1, and hemopoietin-2.  The mononuclear phagocyte
lineage specific growth factor, CSF-1, has been shown to consist of two
disulfide-bonded, 14,000 Mr polypeptide chains that are possibly
identical.  The dimeric protein core is heavily glycosylated with N-linked
oligosaccharides of the acidic "complex" type.  More than 85% of the
carbohydrate can be removed without loss of biological, receptor-binding,
or antibody-binding activities.  However, mild reduction in the absence of
dissociating agents results in loss of these activities.  These properties
of CSF-1 are shared by both murine and human forms of the molecule.  In
collaboration with others, the sequence of the first 39 amino acids at the
N-terminal end of murine CSF-1 has been determined, together with an
internal sequence of 23 additional residues.  There is a high degree of
homology between the human and murine CSF-1 sequences.  The hemopoietins
have been identified as growth factors that regulate the production of
macrophage progenitor cells from developmentally earlier cells.
Hemopoietin-2 is active, by itself, on developmentally later cells than
those responsive to hemopoietin-1.  It has been highly purified from the
serum-free medium conditioned by a murine cell line and is similar in
properties to the factor posseasing erythroid burst promoting activity and
to interleukin 3.  Hemopoietin-1 requires CSF-1 in order to generate
macrophage progenitor cells.  It has been purified from the serum-free
medium conditioned by a human cell line.  Both hemopoietins appear to be
multilineage, rather than lineage specific, hemopoietic growth factors.
(MB)

## Key facts

- **NIH application ID:** 3170439
- **Project number:** 5R01CA032551-08
- **Recipient organization:** ALBERT EINSTEIN COLLEGE OF MEDICINE
- **Principal Investigator:** E. RICHARD STANLEY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $261,437
- **Award type:** 5
- **Project period:** 1982-02-01 → 1992-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170439

## Citation

> US National Institutes of Health, RePORTER application 3170439, HEMOPOIETIC STEM CELL DIFFERENTIATION TO MACROPHAGES (5R01CA032551-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170439. Licensed CC0.

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