# PRODUCTION OF NATURAL KILLER CELLS

> **NIH NIH R01** · UNIVERSITY OF WASHINGTON · 1991 · $187,801

## Abstract

The hypothesis to be tested is that the production of natural killer
(NK) cells is a dynamic developmental process analogous to the
production of other myeloid and lymphoid lineages and is
dependent upon the interactions of three components: NK lineage
progenitor cells, stromal cells, and growth factors.  The aims of
this proposal are to develop the methods and cell lines necessary
to analyze NK cell production and maturation in vitro and to use
those methods to answer fundamental questions about the control
of production of NK cells from their progenitors.  Long and short
term bone marrow cultures will be used to analyze the
requirements for progenitor cells (obtained either from bone
marrow by Percoll gradient centrifugation, positive and negative
selection with antibodies or from established lines of progenitor
cells), stromal cells (cultured whole bone marrow stroma or
selected lines) and growth factors (IL-2, IL-3, IL-1, IL-4 and other
factors (NK-CSF?) found in Con A supernatant.  Limiting dilution
assays will be used to assess the effects on NK lineage cells of the
neutrophilia-inducing tumor CE1460 and the biological response
modifiers AIPP (2-amino-5-iodo-6-phenyl-4-pyrimidinone) and
OK-432 (a pharmaceutical preparation of Streptococcus
pyogenes).  Transforming oncogenes (ab1, myc, and myc/raf) will
be used to infect selected populations of NK lineage cells to
obtain immortalized lines of NK cells and their progenitors.  The
long range goal of these studies is to understand the roles of NK
cells both as products and as regulators of hemopoiesis.

## Key facts

- **NIH application ID:** 3170449
- **Project number:** 5R01CA032553-10
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **SAM.gov UEI:** HD1WMN6945W6
- **Principal Investigator:** SYLVIA B POLLACK
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $187,801
- **Award type:** 5
- **Project period:** 1981-09-01 → 1993-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170449

## Citation

> US National Institutes of Health, RePORTER application 3170449, PRODUCTION OF NATURAL KILLER CELLS (5R01CA032553-10). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170449. Licensed CC0.

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