# NEUROONCOGENESIS BY RESORPTIVE CARCINOGENS

> **NIH NIH R01** · MICHIGAN STATE UNIVERSITY · 1988 · $138,353

## Abstract

In continuing efforts to elucidate the mechanism of pathogenesis
of neoplasia using the N-nitroso tumor model, this proposal deals
with modulation of the transformed state in anaplastic glioma cell
line T9 established from one of the N-nitrosourea-induced tumors.
Nerve growth factor (NGF) and glia maturation factor (GMF) have
been found to induce changes of growth pattern as well as
morphological differentiation of T9 cells in culture.  This system
will be utilized in this project to gain understanding of the
mechanisms of NGF and GMF actions, which may provide the
basis for the potential use of these physiological factors as
reverse transforming agents in cancer therapy.

The role of second messengers and their balance in reverse
transformation will be studied by examining effects of bromo-
cAMP, 1-oleoyl-2-acetylglycerol, 12-tetradecanoylphorbol-13-
acetate, and Ca ionophore as well as effectors of second
messenger production such as cholera toxin, forskolin and
phosphatidic acid on growth differentiation of T9 cells.
Expression of the cellular src gene and synthesis and
phosphorylation of the src gene product as affected by NGF and
GMF as well as various second messengers will be investigated in
order to determine the relationship between reverse
transformation of T9 cells and this gene product which is known
to be closely associated with neural differentiation.  Synthesis and
phosphorylation of tubulin, intermediate filament proteins, actin
and their associated proteins will be examined in T9 cells during
exposure to NGF and GMF as well as various second messengers.
Attempts will then be made to correlate changes in subcellular
organization of cytoskeletons with NGF- and GMF-induced
morphological differentiation.

## Key facts

- **NIH application ID:** 3170490
- **Project number:** 2R01CA032594-06
- **Recipient organization:** MICHIGAN STATE UNIVERSITY
- **SAM.gov UEI:** R28EKN92ZTZ9
- **Principal Investigator:** ADALBERT KOESTNER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $138,353
- **Award type:** 2
- **Project period:** 1981-10-01 → 1990-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170490

## Citation

> US National Institutes of Health, RePORTER application 3170490, NEUROONCOGENESIS BY RESORPTIVE CARCINOGENS (2R01CA032594-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170490. Licensed CC0.

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