# GLYCOCONJUGATE STRUCTURE AND FUNCTION IN LEUKOCYTES

> **NIH NIH R01** · MEDICAL RESEARCH INSTITUTE OF SAN FRAN · 1988 · $150,124

## Abstract

The carbohydrate portions of cell membrane glycolipids and glycoproteins
are important in both the function and immunologic recognition of cells.
Both cell-\and tissue-specific complex carbohydrates exist and many
cell-\and tissue-specific monoclonal antibodies are found to be directed
against these moeities.  The aims of this work are to isolate and
chemically characterize these complex carbohydrate moeities of human white
blood cell membrane glycolipids and glycoproteins and to determine their
immunologic and functional roles.  This will be accomplished by acquiring
large numbers of cells from leukapheresis of normal persons and persons
with various leukemias. Isolation of the molecules will be done and
chemical analysis will be performed using column chromatography, gas-liquid
and high-pressure liquid chromatography and mass spectrometry.  Enzyme
hydrolysis will be done to confirm the sequences.  Immunologic studies will
be done using polyclonal and monoclonal antibodies directed against normal
and leukemic cells, and methods will include immunoblotting, radial
immunodiffusion and cytofluorometry.  Functional studies will be done
utilizing isolated glycopeptides and glycolipids (and antibodies to them)
in several in vitro systems such as assays for adhesion, killing, oxygen
radical generation, and phagocytosis for granulocytes.  For lymphoid cells
aasays will include lymphokine production, allogeneic immunologic response,
and phenotypic typing.  These studies are important to our understanding of
the nature of leukocyte and leukemia-specific antigens and to the functions
of human leukocyte cell surface glycoproteins.  They will also greatly
improve our understanding of the role that surface glycoproteins and
glycolipids play in the development and maintenance of the leukemia cell
phenotype.  (AG)

## Key facts

- **NIH application ID:** 3170528
- **Project number:** 5R01CA032632-07
- **Recipient organization:** MEDICAL RESEARCH INSTITUTE OF SAN FRAN
- **Principal Investigator:** JOHN C KLOCK
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $150,124
- **Award type:** 5
- **Project period:** 1984-12-01 → 1989-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170528

## Citation

> US National Institutes of Health, RePORTER application 3170528, GLYCOCONJUGATE STRUCTURE AND FUNCTION IN LEUKOCYTES (5R01CA032632-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170528. Licensed CC0.

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