# ROLE OF PAPILLOMAVIRUS IN CERVICAL NEOPLASIA

> **NIH NIH R01** · GEORGETOWN UNIVERSITY · 1987 · $167,929

## Abstract

Recent molecular virologic and immunologic studies have shown a
relationship between human papillomavairus (HPV) infection and dysplasia of
the uterine cervix.  The significance of these observations is underscored
by the fact that dysplasias are considered precursor lesions to cervical
carcinoma.  The specific aims of this proposal are to characterize HPV
sequences from dysplasias by molecular cloning for the purpose of
identifying the various HPV types associated with these lesions using
molecular hybridization and immunological tests with antibodies directed
against virus type-specific poly- or oligopeptides.

HPV sequences will be cloned into plasmid or Charon vectors and
subsequently characterized by hybridization to DNAs of known HPV types.
These cloned DNAs will be used  in blot transfer and in situ hybridization
experiments with dysplasias and cervical carcinomas.  Sequence analysis of
the viral DNAs will provide necessary information for the synthesis of
virus type-specific structural polypeptides in bacterial expression vector
systems or synthesis of oligopeptides by solid-phase chemistry.  The
resultant antibodies will be used to screen biopsy specimens to determine
the HPV type associated with dysplasias which regress and those which
progress to more serious disease.  Antibodies directed against
papillomavirus genus-specific nonstructural (transforming) proteins will be
synthesized in bacteria for use in immunocytochemical studies on cervical
cancers.

From the behavior of cervical dysplasia (regression or progression) it
would be of prognostic and/or diagnostic value to be able to predict the
outcome of HPV infection of the cervix.  Such information could provide a
more rational approach to the management of cervical neoplasia.

## Key facts

- **NIH application ID:** 3170531
- **Project number:** 2R01CA032638-06A1
- **Recipient organization:** GEORGETOWN UNIVERSITY
- **SAM.gov UEI:** TF2CMKY1HMX9
- **Principal Investigator:** WAYNE D LANCASTER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $167,929
- **Award type:** 2
- **Project period:** 1984-01-01 → 1992-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170531

## Citation

> US National Institutes of Health, RePORTER application 3170531, ROLE OF PAPILLOMAVIRUS IN CERVICAL NEOPLASIA (2R01CA032638-06A1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170531. Licensed CC0.

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