# STRUCTURE FUNCTION RELATIONSHIP OF ANTIESTROGEN

> **NIH NIH R01** · UNIVERSITY OF WISCONSIN-MADISON · 1991 · $154,014

## Abstract

Tamoxifen, a nonsteroidal antiestrogen, is available to treat all
stages of breast cancer and is being considered as a preventive for breast
cancer in high risk women.  The drug has been shown to produce a survival
advantage in node positive postmenopausal women and to exhibit a low
incidence of side effects.  The broad application of tamoxifen in the
clinic requires not only a clear understanding of the mode of action of
antiestrogens but also an understanding of drug resistance.  The goal of
this proposal is to understand the structural requirements for estrogen and
antiestrogen regulated growth of breast cancer cells in culture.  Our study
of structure activity relationships of novel nonsteroidal compounds will
be completed to provide tools to compare and contrast the changes in growth
factor (transforming growth factors alpha, beta1, beta2, beta3) growth
factor receptor (epidermal growth factor) and steroid hormone receptors
(estrogen and progesterone) in MCF-7 and T47D breast cancer cells during
replication.  New pure antiestrogens (e.g., ICI164,384) may provide a new
dimension for the treatment of breast cancer.  However, we are naive about
the effects that complete estrogen withdrawal will have on the tumor.  We
have developed several estrogen non-responsive varieties of breast cancer
cells (MCF-7 and T47D) by long-term estrogen deprivation.  One clone of
T47D cells (C4) is of particular interest as it is refractory to estrogens
and antiestrogens and appears to have lost the mechanism for both estrogen
and progesterone receptor induction.  Our aim is to understand receptor and
growth factor regulation in antiestrogen resistant breast cancer cells so
that novel treatment strategies can be developed to treat patients when
antiestrogen resistance occurs in the clinic.

## Key facts

- **NIH application ID:** 3170575
- **Project number:** 2R01CA032713-10
- **Recipient organization:** UNIVERSITY OF WISCONSIN-MADISON
- **SAM.gov UEI:** LCLSJAGTNZQ7
- **Principal Investigator:** VIRGIL CRAIG JORDAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $154,014
- **Award type:** 2
- **Project period:** 1982-08-01 → 1994-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170575

## Citation

> US National Institutes of Health, RePORTER application 3170575, STRUCTURE FUNCTION RELATIONSHIP OF ANTIESTROGEN (2R01CA032713-10). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170575. Licensed CC0.

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