# SKIN TUMORIGENESIS STUDIED WITH CELL MARKERS

> **NIH NIH R01** · UNIVERSITY OF WASHINGTON · 1987 · $175,258

## Abstract

The primary goal of this proposal is to learn more about early
events in epithelial carcinogenesis and the factors that are
related to progression of premalignant proliferations to overt
malignancy.  The experimental model to be used is two-stage
carcinogen-induced skin tumors in female mice with X-
chromosome linked phosphoglycerate kinase (PGK) cell markers.
Specifically, we will attempt to answer the following questions
about mouse skin tumor development in the hope of further
understanding human tumorigenesis:  Do spontaneously-occurring
mouse skin papillomas progress sequentially to carcinomas?  Can
promotor-dependent papillomas induced by very low doses of
DMBA initiation and a standard dose of TPA promotion be
advanced to promoter-independent papillomas or carcinomas by
increasing the dose of TPA or by increasing the length of
promotion?  In mice initiated with high doses of DMBA, does a
decrease in the dose of TPA, a decrease in the length of
promotion or substitution of a weak promoter for TPA during the
first course of promotion result in the induction of papillomas
which are primarily promoter-independent?  Do papillomas
induced by initiation with weak carcinogens and TPA promotion
change their clonal phenotype during progression as occurs in
those induced by DMBA and TPA?  What is the mechanism of
regression of promoter-dependent papillomas?  These experiments
should permit further development of a model of carcinogenesis
that may be useful in investigation of molecular mechanisms of
oncogenesis and could suggest a better means of approaching
study of epithelial carcinogenesis in humans.

## Key facts

- **NIH application ID:** 3170583
- **Project number:** 2R01CA032716-04A1
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **SAM.gov UEI:** HD1WMN6945W6
- **Principal Investigator:** ARRAM L REDDY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $175,258
- **Award type:** 2
- **Project period:** 1983-07-01 → 1990-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3170583

## Citation

> US National Institutes of Health, RePORTER application 3170583, SKIN TUMORIGENESIS STUDIED WITH CELL MARKERS (2R01CA032716-04A1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3170583. Licensed CC0.

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